PXD033808

PXD033808 is an original dataset announced via ProteomeXchange.

Title	SerpinB2/TPPII regulates senescence
Description	Tripeptidyl peptidase II (TPPII) degrades N-terminal tripeptides from proteins and peptides. Studies in both human and mice have shown that TPPII deficiency is linked to cellular immune-senescence, lifespan regulation, and the aging process. However, the mechanism of how TPPII participates in these processes is less clear. In this study, we established a chemical probe-based assay and found that while the mRNA and protein levels of TPPII were not altered during senescence, its enzymatic activity was reduced in senescent human fibroblasts. We also showed that elevation of serine protease inhibitor serpinB2 reduced TPPII activity in senescent cells. Moreover, suppression of TPPII led to elevation of lysosomal contents as well as TPPI and -galactosidase activities, suggesting that the lysosome biogenesis is induced to compensate for the reduction of TPPII activity in senescent cells. Together this study discloses a critical role of the serpinB2/TPPII signaling pathway in proteostasis during senescence. Since serpinB2 level can be increased by a variety of cellular stresses, reduction of TPPII activity through activation of serpinB2 might represent a common pathway for cells to respond to different stress conditions.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_00:29:21.713.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033808
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yi-Ju Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-11 05:56:52	ID requested
⏵ 1	2022-05-31 00:29:22	announced

Liao CL, Hu RC, Liao MS, Chen YJ, Chen YP, Hsieh HH, Tai CH, Chou TC, Chu CY, Chen YJ, Lo LC, Lin JJ, Unveiling a novel serpinB2-tripeptidyl peptidase II signaling axis during senescence. J Cell Sci, 135(10):(2022) [pubmed]

submitter keyword: activity-based probe

serine hydrolase

TPP

senescence

benzylphosphonofluoridate

Yi-Ju Chen
contact affiliation	Institute of Chemistry, Academia Sinica, Taiwan
contact email	tp7249@gate.sinica.edu.tw
lab head
Yi-Ju Chen
contact affiliation	Academia Sinica
contact email	tp7249@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033808
     1. Label: PRIDE project
     2. Name: SerpinB2/TPPII regulates senescence