PXD033799

PXD033799 is an original dataset announced via ProteomeXchange.

Title	Cryptococcus neoformans Hsf3 LC-MS/MS
Description	Mitochondrial quality control is essential in modulating intramitochondrial ROS (mtROS) homeostasis that is a critical determinant for many human disorders. Here we identified an atypical function of a heat shock factor, Hsf3, as an important mtROS regulator. Despite HSFs are nuclear proteins and master regulators of protein unfolded response (UPR) across taxa, we demonstrate that Hsf3 is a both nuclei and mitochondria targeting transcription factor that plays an irrelevant function in controlling UPR process. Instead, Hsf3 represses TCA cycle genes and simultaneously regulates electron transfer chain genes encoded from both nuclei and mitochondria. Moreover, both nuclear and mitochondrial targeting signal are required for promising Hsf3 function. Hsf3 regulation responds to multiple intramitochondrial stresses, which activates and ameliorates Hsf3 DNA binding via oxidation of the cysteine residue on DNA binding domain of Hsf3. Collectively, our findings reveal a previously unknown role of HSF family in modulation of mitochondrial function and damage.
HostingRepository	PRIDE
AnnounceDate	2022-09-01
AnnouncementXML	Submission_2022-08-31_20:13:09.652.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xindi Gao
SpeciesList	scientific name: Cryptococcus neoformans var. grubii H99; NCBI TaxID: 235443;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-11 02:25:00	ID requested
⏵ 1	2022-08-31 20:13:10	announced

Dataset with its publication pending

submitter keyword: Cryptococcus neoformans, Mitochondrial

Chen Ding
contact affiliation	Northeastern University
contact email	dingchen@mail.neu.edu.cn
lab head
Xindi Gao
contact affiliation	Northeastern University
contact email	gaoxindi123@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033799
     1. Label: PRIDE project
     2. Name: Cryptococcus neoformans Hsf3 LC-MS/MS