PXD033797

PXD033797 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	TurboID-mediated proximal labeling of Drosophila BubR1 and its cleavage variants
Description	V5-TurboID-tagged Drosophila BubR1 and its cleavage variants were transiently expressed in Drosophila S2 cells in two biological replicates. Cells were treated with 500 µM biotin for 30 min for protein biotinylation. Biotinylated protein-containing lysate was subjected to FG-NeutrAvidin beads purification followed by on-beads trypsin digestion. Digested peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.
HostingRepository	jPOST
AnnounceDate	2023-05-11
AnnouncementXML	Submission_2023-05-10_08:00:06.244.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natsuki Shinoda
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	L-cysteine methyl disulfide; alpha-amino acetylated residue; L-methionine sulfoxide; ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-10 23:19:25	ID requested
⏵ 1	2023-05-10 08:00:06	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: BubR1, Caspase, Drosophila, TuboID, LFQ

submitter keyword: BubR1, Caspase, Drosophila, TuboID, LFQ

Contact List

Masayuki Miura
lab head
Natsuki Shinoda
contact affiliation	The University of Tokyo
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001582/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001582/