PXD033788

PXD033788 is an original dataset announced via ProteomeXchange.

Title	Proteomics and liquid biopsy characterization of human EMT-related metastasis in colorectal cancer
Description	Tumor cells undergo epithelial-mesenchymal transition (EMT), however, there is a room of disagreement in role of EMT heterogeneity to colorectal cancer metastasis (mCRC) evolution. To uncover new EMT-related metastasis proteins and pathways, we addressed the EMT status in colorectal cancer liver metastasis patient-derived CTCs to identify proteins that promote their distant metastasis. And then, we performed a comparative proteomic analysis in matched pairs of primary tumor tissues, adjacent mucosa tissues and liver metastases tissues. By integrative analysis we show that, unstable Epithelial/Mesenchymal (E/M)-type CTCs had the strongest liver metastases formation ability and the proportion of E/M-type CTCs correlated with distant metastases. Using an optimized proteomic workflow including data independent acquisition (DIA) and parallel reaction monitoring (PRM), we identified novel EMT-related protein cluster (e.g., GNG2, COL6A1, COL6A2, DCN, COL6A3, LAMB2, TNXB, CAVIN1) and well-described (e.g., KRAS and ERBB2) core protein level changes in EMT-related metastasis progression, and the proteomic data indicate KRAS, ERBB2, COL6A1 and CAVIN1 are promising EMT-related metastatic biomarker candidates. This study contributes to catch on EMT in CRC metastasis and in favor of heterogeneous EMT phenotypes as a key piece for tumor outcome and treatment. We further propose that therapies targeting this aggressive subset (E/M-type) of CTCs and related protein may be worthy of exploration as potential suppressors of metastatic evolution
HostingRepository	iProX
AnnounceDate	2022-05-09
AnnouncementXML	Submission_2023-08-14_00:32:15.797.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Linhai Yan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-09 18:19:56	ID requested
1	2022-06-30 19:25:00	announced
⏵ 2	2023-08-14 00:32:16	announced	2023-08-14: Update publication information.

Huang M, Wu Y, Cheng L, Fu L, Yan H, Ru H, Mo X, Yan L, Su Z, Multi-omics analyses of glucose metabolic reprogramming in colorectal cancer. Front Immunol, 14():1179699(2023) [pubmed]

submitter keyword: mCRC, Epithelial-mesenchymal transition, Proteomics, Liquid biopsy, COL6A1

Linhai Yan
contact affiliation	Guangxi Medical University Cancer Hospital
contact email	yanlinhai000@163.com
lab head
Linhai Yan
contact affiliation	Guangxi Medical University Cancer Hospital
contact email	yanlinhai000@163.com
dataset submitter

iProX dataset URI