PXD033782

PXD033782 is an original dataset announced via ProteomeXchange.

Title	PROTEOMICS REVEALS DIFFERENTIALLY REGULATED PATHWAYS WHEN COMPARING LOW-VERSUS HIGH-GRADE ASTROCYTOMAS
Description	Astrocytic tumors are known for their high progression capacity and for high mortality rates; in this regard, proteins correlated to prognosis can aid medical conduct. Although several genetic changes related to progression from low to high-grade astrocytoma are already known, mRNA copies do not necessarily correlate with protein abundance and, therefore could shadow further comprehension about this tumor’s biology. This motivates us to seek for complementary strategies to study tumor progression at the protein level. Here we compare the proteomic profile of biopsies from patients with low-grade (diffuse, n=6) versus high-grade astrocytoma (anaplastic and glioblastomas, n =10), using shotgun proteomics. Data analysis performed with PatternLab for proteomics identified 5,206 and 6,004 proteins in the low- and high-grade groups, respectively. Our results revealed seventy-four differentially abundant proteins (p < 0.01); we then shortlist those related to greater malignancy. We also describe molecular pathways distinctly activated in the two groups, such as differences in the organization of the extracellular matrix, decisive both in tumor invasiveness and in signaling for cell division, which, together with marked contrasts in energy metabolism, are determining factors in the speed of growth and dissemination of these neoplasms. The degradation pathways of GABA, enriched in the low-grade group, is consistent with a favorable prognosis. Other functions such as platelet degranulation, apoptosis, and activation of the MAPK pathway were correlated to high-grade tumors and, consequently, unfavorable prognoses. Our results provide an important survey of molecular pathways involved in glioma pathogenesis for these histopathological groups.
HostingRepository	PRIDE
AnnounceDate	2023-08-04
AnnouncementXML	Submission_2023-08-04_10:50:57.193.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MarlonD M Santos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-09 05:38:03	ID requested
⏵ 1	2023-08-04 10:50:57	announced

Dataset with its publication pending

submitter keyword: mass spectrometer, low-grade astrocytoma and high-grade astrocytoma.,Proteomics

Juliana de S. da G.Fischer
contact affiliation	Laboratory for structural and computational proteomics - Carlos Chagas Institute, Fiocruz Paraná, Brazil
contact email	julifr@gmail.com
lab head
MarlonD M Santos
contact affiliation	Computational Mass Spectrometry & Proteomics Group - Fiocruz
contact email	marlondms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033782
     1. Label: PRIDE project
     2. Name: PROTEOMICS REVEALS DIFFERENTIALLY REGULATED PATHWAYS WHEN COMPARING LOW-VERSUS HIGH-GRADE ASTROCYTOMAS