PXD033765

PXD033765 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	De novo mapping of the apicomplexan Ca2+-responsive phosphoproteome
Description	Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition between the replicative and lytic phases of the infectious cycle. Despite the presence of conserved Ca2+-responsive proteins, little is known about how specific signaling elements interact to impact pathogenesis. We mapped the Ca2+-responsive proteome of the model apicomplexan T. gondii via time-resolved phosphoproteomics. The waves of phosphoregulation following PKG activation and stimulated Ca2+ release corroborate known physiological changes but identify specific molecular targets in these pathways. We characterized protein phosphatase 1 (PP1) as a Ca2+-responsive enzyme that relocalized to the parasite apex upon Ca2+ store release. Conditional depletion of PP1 revealed that the phosphatase regulates Ca2+ uptake to promote parasite motility. PP1 may thus be partly responsible for Ca2+-regulated serine/threonine phosphatase activity in apicomplexan parasites.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:32:36.693.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033765
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alice Herneisen
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-08 04:16:02	ID requested
1	2022-10-14 22:41:15	announced
⏵ 2	2023-11-14 07:32:38	announced	2023-11-14: Updated project metadata.

Publication List

Herneisen AL, Li ZH, Chan AW, Moreno SNJ, Lourido S, -responsive pathways. Elife, 11():(2022) [pubmed]
10.6019/PXD033765;

Herneisen AL, Li ZH, Chan AW, Moreno SNJ, Lourido S, -responsive pathways. Elife, 11():(2022) [pubmed]

10.6019/PXD033765;

Keyword List

submitter keyword: Toxoplasma, parasite, calcium signaling, phosphoproteomics, apicomplexan, systems biology

submitter keyword: Toxoplasma, parasite, calcium signaling, phosphoproteomics, apicomplexan, systems biology

Contact List

Sebastian Lourido
contact affiliation	Associate Professor, Department of Biology, Massachusetts Institute of Technology
contact email	lourido@wi.mit.edu
lab head
Alice Herneisen
contact affiliation	Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology
contact email	alice@wi.mit.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD033765
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD033765

PRIDE project URI

Repository Record List

[ + ]