PXD033756

PXD033756 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Segal_14-3-3_interactors_mutants_BioID_P77_VS19
Description	This dataset consists of 37 raw MS files and associated peak lists and result files, acquired on TripleTOF6600 mass spectrometer operated in Data Dependent Acquisition mode. The files are associated with a manuscript submitted for publication by Segal et al. that determines the protein-protein and proximity interaction landscape of 14-3-3 proteins. This particular dataset consists in files associated with three baits (14-3-3 interactors) profiled in both a wild type and a 14-3-3 binding site mutant. Each bait was profiled in biological duplicates by BioID (*using the BioID2 enzyme), alongside a total of 25 negative controls (used for analysis with SAINTexpress). See details within the README file. Samples were generated by Dmitri Segal and affinity purification and mass spectrometry were performed by Zhen-Yuan Lin with oversight from Brett Larsen and Anne-Claude Gingras. Analysis was performed by Anne-Claude Gingras and Mikko Taipale. Corresponding authors: Mikko Taipale (mikko.taipale@utoronto.ca)/lead author and Anne-Claude Gingras (gingras@lunenfeld.ca). Anne-Claude Gingras should be contacted for questions on this dataset.
HostingRepository	MassIVE
AnnounceDate	2022-05-07
AnnouncementXML	Submission_2022-05-07_06:28:30.465.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gingras Lab
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human adenovirus 5; NCBI TaxID: 28285;
ModificationList	deamidated L-asparagine; deamidated L-glutamine; L-methionine sulfoxide
Instrument	TripleTOF 6600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-07 03:24:43	ID requested
⏵ 1	2022-05-07 06:28:30	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: BioID, Proximity-dependent biotinylation, 14-3-3 proteins, P-body, Stress granules

submitter keyword: BioID, Proximity-dependent biotinylation, 14-3-3 proteins, P-body, Stress granules

Contact List

Anne-Claude Gingras
contact affiliation	LTRI
contact email	gingras@lunenfeld.ca
lab head
Gingras Lab
contact affiliation	Samuel Lunenfeld Research Institute
contact email	gingras@mshri.on.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089437/