PXD033734

PXD033734 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of affinity-purified praja2 complexes
Description	Praja2 is a RING E3 ubiquitin ligase, widely expressed in mammalian cells and tissues which is involved in essential aspects of cell physiology. Several signaling pathways are regulated by praja2. The stability of regulatory PKA subunits is directly regulated by Praja2, which binds and tethers cAMP-dependent protein kinase (PKA) to intracellular membranes and organelles, ensuring efficient integration, propagation and amplification of the locally-generated cAMP to distinct target compartments. By regulating cAMP signaling, praja2 efficiently couples phosphorylation to ubiquitination of protein kinases, scaffolds and effectors, with important implication for neuronal activity, development, inflammatory responses, ciliogenesis, cell growth and metabolism. Dysregulation of praja2-regulated signaling pathways has been causally linked to the growth and progression of glioblastoma.
HostingRepository	PRIDE
AnnounceDate	2026-06-25
AnnouncementXML	Submission_2026-06-25_08:11:31.246.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CHIARA D'AMBROSIO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-06 03:32:05	ID requested
⏵ 1	2026-06-25 08:11:31	announced

10.1038/S42003-022-03639-8;

submitter keyword: LC-MS-MS,praja2, interactome

Andrea Scaloni
contact affiliation	Proteomics, Metabolomics & Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment (ISPAAM), National Research Council, Piazzale Enrico Fermi 1, 80055 Portici (NA), Italy
contact email	andrea.scaloni@cnr.it
lab head
CHIARA D'AMBROSIO
contact affiliation	ISPAAM CNR
contact email	chiara.dambrosio@cnr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD033734

PRIDE project URI

• PRIDE
  1. PXD033734
     1. Label: PRIDE project
     2. Name: Proteomic analysis of affinity-purified praja2 complexes