PXD033732

PXD033732 is an original dataset announced via ProteomeXchange.

Title	Anastellin impacts on the processing of extracellular matrix and stimulates the release of cytokines from smooth muscle cells
Description	Anastellin, a recombinant protein fragment from the first type III module of fibronectin, mimics a partially-unfolded intermediate with exposed cryptic protein-protein interaction sites implicated in assembly of fibronectin fibrils. Anastellin initiates in vitro fibrillation of fibronectin, yielding “superfibronectin”, a polymer with enhanced cell-adhesive properties. This ability is absent in an anastellin double mutant, L37AY40A. Here we demonstrate that both wild-type and L37AY40A anastellin affects fibronectin processing within the ECM of smooth muscle cells. FN fibrils are diminished in the ECM from cells treated with anastellin, but are partially rescued by supplementation with plasma fibronectin in cell media. Proteomics analysis reveals that anastellin also impacts on the processing of other ECM proteins, with increased collagen and decreased laminin detected in media from cells exposed to wild-type anastellin.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:10.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Per Hägglund
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-06 03:31:32	ID requested
1	2023-02-01 03:07:59	announced
⏵ 2	2023-11-14 08:28:14	announced	2023-11-14: Updated project metadata.

He J, Steffen JH, Thulstrup PW, Pedersen JN, Sauerland MB, Otzen DE, Hawkins CL, Gourdon P, Davies MJ, H, ä, gglund P, Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells. Sci Rep, 12(1):22051(2022) [pubmed]

submitter keyword: Anastellin, fibronectin, smooth muscle cells, extracellular matrix

Per Hägglund
contact affiliation	1Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
contact email	hagglundperm@gmail.com
lab head
Per Hägglund
contact affiliation	University of Copenhagen
contact email	hagglundperm@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD033732

PRIDE project URI

• PRIDE
  1. PXD033732
     1. Label: PRIDE project
     2. Name: Anastellin impacts on the processing of extracellular matrix and stimulates the release of cytokines from smooth muscle cells