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PXD033704

PXD033704 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntracellular proteome response of the gut bacterium Bacteroides thetaiotaomicron to the metabolic switch from glucose to sucrose as the sole carbon source
DescriptionBacteroides thetaiotaomicron (B. thetaiotaomicron) is an essential bacterial representative of the human gut microbiome that metabolizes a wide range of dietary and mucosal polysaccharides. Here, we analyze the intracellular proteomic response of in vitro grown B. thetaiotaomicron to the metabolic switch from glucose to sucrose as the sole carbon source. To determine the effects of nutrient metabolism, we applied two LC-based quantitative proteomics approaches encompassing label free quantification (LFQ) and isobaric labeling by tandem mass tags (TMT). The obtained results were compared with respect to the number of identified and quantified proteins, peptides supporting identification and quantification, sequence coverage, reproducibility, and statistical findings. A total of 1719 and 1696 proteins were quantified, covering 35 % of the predicted B. thetaiotaomicron proteome. Both approaches provide a high degree of quantitative and qualitative data, showing that sucrose molecules are metabolized using enzymes and transport systems encoded by a specific polysaccharide utilization locus for fructan metabolism.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:35:35.025.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Tholey
SpeciesList scientific name: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482); NCBI TaxID: 226186;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-05 01:12:32ID requested
12022-10-14 22:56:48announced
22023-11-14 07:35:37announced2023-11-14: Updated project metadata.
Publication List
Genth J, Kaleja P, Treitz C, Sch, ä, fer K, Graspeuntner S, Rupp J, Tholey A, The intracellular proteome of the gut bacterium Bacteroides thetaiotaomicron is widely unaffected by a switch from glucose to sucrose as main carbohydrate source. Proteomics, 22(22):e2200189(2022) [pubmed]
Keyword List
submitter keyword: label free quantifications, isobaric labeling, IBD, inflammation, TMT, carbohydrate metabolism, bottom up, LFQ,Bacterial proteomics, high/low pH 2D-LC
Contact List
Andreas Tholey
contact affiliationSystematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, Kiel, Germany
contact emaila.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliationSystematic Proteome Research & Bioanalytics, University of Kiel
contact emaila.tholey@iem.uni-kiel.de
dataset submitter
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