PXD033699

PXD033699 is an original dataset announced via ProteomeXchange.

Title	The novel type II toxin-antitoxin PacTA modulates Pseudomonas aeruginosa iron homeostasis by obstructing the DNA-binding activity of Fur
Description	Type II Toxin-antitoxin (TA) systems are widely distributed in bacterial and archaeal genomes with diverse critical cellular functions such as defense against phages, biofilm formation, persistence and virulence. GCN5-related N -acetyltransferase (GNAT) toxin, with an acetyltransferase activity-dependent mechanism of translation inhibition, represents a relatively new and expanding family of type II TA toxins. Here, we describe a group of GNAT-Xre TA modules that are widely distributed among Pseudomonas species and even certain Gram-positive bacteria. We investigate one of its members PacTA (encoded by PA3270/PA3269) from Pseudomonas aeruginosa, and demonstrate that the toxin PacT positively regulates the iron acquisition in P. aeruginosa. Notably, other than arresting translation via acetylating aminoacyl-tRNAs, PacT could directly bind to Fur, a key ferric uptake regulator, to attenuate its DNA-binding affinity and thus permit expression of downstream iron-acquisition-related genes. We further show that expression of the pacTA locus is up-regulated in response to iron starvation and the absence of PacT causes biofilm formation defect and attenuated pathogenesis. Overall, these findings reveal a novel regulation mechanism of GNAT toxin that controls genes involved in iron uptake process and contributes to the bacterial virulence.
HostingRepository	iProX
AnnounceDate	2022-05-05
AnnouncementXML	Submission_2023-01-28_18:24:50.421.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Siping Zhang
SpeciesList	scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 01:11:35	ID requested
1	2022-05-12 04:12:00	announced
⏵ 2	2023-01-28 18:24:51	announced	2023-01-29: Update publication information.

submitter keyword: Type II toxin-antitoxin systems, Pseudomonas aeruginosa, iron homeostasis , DNA-binding activity

Yongxing He
contact affiliation	Lanzhou University
contact email	heyx@lzu.edu.cn
lab head
Siping Zhang
contact affiliation	Lanzhou University
contact email	zhangsp2015@lzu.edu.cn
dataset submitter

iProX dataset URI