PXD033697

PXD033697 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of early stages of human osteoblasts osteogenic differentiation in vitro
Description	Cellular mechanisms and signaling cascades underlying the early stages of osteoblast progenitors differentiation in adult bone are still not well understood. Therefore, we performed shotgun proteomics analysis of primary culture of isolated human osteoblasts from femur of adult donors in control and on the sixth day of osteogenic differentiation in vitro. This is an early timepoint in which we have observed no extracellular matrix mineralization yet. Nevertheless, for protein extraction we used acidic extraction method which is effective for bones and other mineralized tissues. 1785 proteins identified with at least two unique peptides were included in proteomics analysis. We revealed physiological shift associated with a decrease of cells proliferative activity and extracellular matrix secretion and organization. The obtained data can make a significant contribution to understanding processes of osteogenesis induction to enhance fracture healing.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:34:33.444.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033697
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arseniy Lobov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 01:11:07	ID requested
1	2022-12-25 04:18:05	announced
⏵ 2	2023-11-14 07:34:33	announced	2023-11-14: Updated project metadata.

10.6019/PXD033697;

submitter keyword: osteogenic differentiation, PASEF, shotgun proteomics, TimsToF Pro,osteoblasts, DDA

Anna B. Malashicheva
contact affiliation	Institute of cytology of the Russian Academy of Science
contact email	amalashicheva@gmail.com
lab head
Arseniy Lobov
contact affiliation	Emmanuel Levy Lab, Dept. of Chemical and Structural Biology, Weizmann Institute of Science
contact email	arseniylobov@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033697
     1. Label: PRIDE project
     2. Name: Proteomics analysis of early stages of human osteoblasts osteogenic differentiation in vitro