PXD033693

PXD033693 is an original dataset announced via ProteomeXchange.

Title	Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families
Description	Endocytic recycling controls the return of internalised cargos to the plasma membrane to coordinate their positioning, availability and downstream signalling. The Rab4 and Rab11 small GTPase families regulate distinct recycling routes, broadly classified as fast recycling from early endosomes (Rab4) and slow recycling from perinuclear recycling endosomes (Rab11), and both routes handle a broad range of overlapping cargos to regulate cell behaviour. We adopted a proximity labelling approach, BioID, to identify and compare the protein complexes recruited by Rab4a, Rab11a and Rab25 (a Rab11 family member implicated in cancer aggressiveness), revealing statistically robust protein-protein interaction networks of well characterised and new cargos and trafficking machinery in migratory cancer cells. Gene ontological analysis of these interconnected networks revealed that these endocytic recycling pathways are intrinsically connected to cell motility and cell adhesion. Using a knock sideways relocalisation approach we were further able to confirm novel links between Rab11/25 and the ESCPE-1 and retromer multiprotein sorting complexes and identify new endocytic recycling machinery associated with Rab4, Rab11 and Rab25 that regulate cancer cell migration in 3D-matrix.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:50.879.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Craig Lawless
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 01:03:47	ID requested
1	2023-06-13 08:26:13	announced
⏵ 2	2023-11-14 09:00:51	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: BioID, Human, Rab11a, Endocytosis, Rab25, Rab4a

Patrick Caswell
contact affiliation	Senior Lecturer, Wellcome Trust Centre for Cell-Matrix Research, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13
contact email	patrick.caswell@manchester.ac.uk
lab head
Craig Lawless
contact affiliation	University of Manchester
contact email	craig.lawless@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD033693

PRIDE project URI

• PRIDE
  1. PXD033693
     1. Label: PRIDE project
     2. Name: Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families