PXD033691
PXD033691 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identifying new interactors of NGN3 through IP-MS |
| Description | NGN3 is a transcription factor whose transient expression during pancreatic development is vital for the generation of endocrine pancreatic cells, including beta cells. NGN3 stabilisation has been shown to induce exocrine-to-endocrine cell plasticity in the murine pancreas, making it a viable target for therapies aiming to replenish beta cells after immune-mediated destruction in type 1 diabetes patients. Here, we set out to identify new interactors of NGN3 that could play a role in its post-translational regulation. We transfected HEK293A cells with HA-tagged NGN3 and carried out immunoprecipitation of the HA-tag, followed by analysis of co-immunoprecipitated interactors via LC-MS/MS. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-04 |
| AnnouncementXML | Submission_2024-10-04_01:40:20.036.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD033691 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Teodora Manea |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-05-05 01:03:29 | ID requested | |
| 1 | 2023-03-27 05:10:08 | announced | |
| ⏵ 2 | 2024-10-04 01:40:20 | announced | 2024-10-04: Updated project metadata. |
Publication List
| 10.1038/s41467-023-38146-9; |
| Manea T, Nelson JK, Garrone CM, Hansson K, Evans I, Behrens A, Sancho R, USP7 controls NGN3 stability and pancreatic endocrine lineage development. Nat Commun, 14(1):2457(2023) [pubmed] |
| 10.6019/PXD033691; |
Keyword List
| ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Diabetes (B/D-HPP), Human Proteome Project, Stems Cells (B/D-HPP) |
| submitter keyword: IP-MS, HA-tag,NGN3, HEK293A, LC-MS/MS |
Contact List
| Rocio Sancho | |
|---|---|
| contact affiliation | 1. Centre for Gene Therapy and Regenerative Medicine, King’s College London, London, UK 2. Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany |
| contact email | rocio.sancho@kcl.ac.uk |
| lab head | |
| Teodora Manea | |
| contact affiliation | King's College London |
| contact email | teodora.manea@kcl.ac.uk |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033691 |
| PRIDE project URI |
Repository Record List
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