PXD033690

PXD033690 is an original dataset announced via ProteomeXchange.

Title	Detoxified synthetic bacterial membrane vesicles as a platform of vaccines against bacteria and SARS-CoV-2
Description	The development of vaccines based on outer membrane vesicles (OMV) that naturally bud off from bacteria is an evolving field in infectious diseases. However, the inherent inflammatory nature of OMV limits their use as human vaccines. This study employed an engineered vesicle technology to develop synthetic bacterial vesicles (SyBV or aOMV) that activate the immune system without the severe immunotoxicity of OMV. SyBV were generated from bacterial membranes through treatment with detergent and ionic stress. SyBV induced less inflammatory responses in macrophages and in mice compared to natural OMV. Immunization with SyBV or OMV induced comparable antigen-specific adaptive immunity. Specifically, immunization with Pseudomonas aeruginosa-derived SyBV protected mice against bacterial challenge, and this was accompanied by significant reduction in lung cell infiltration and inflammatory cytokines. Further, immunization with Escherichia coli-derived SyBV protected mice against E. coli sepsis, comparable to OMV-immunized group. The protective activity of SyBV was driven by the stimulation of B-cell and T-cell immunity. Also, SyBV had the ability to confer cross-protection against different bacterial strain. Next, SyBV were engineered to display the SARS-CoV-2 S1 protein on their surface, and these vesicles induced specific S1 protein antibody and T-cell responses. Collectively, these results demonstrate that SyBV may be a safe and efficient vaccine platform for the prevention of bacterial and viral infections
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:55:06.837.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evelin Berger
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	methylthiolated residue; deaminated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 01:03:29	ID requested
1	2023-07-20 10:56:54	announced
2	2023-11-14 09:02:54	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:55:12	announced	2024-10-22: Updated project metadata.

10.1186/s12951-023-01928-w;

Park KS, Svennerholm K, Crescitelli R, L, ä, sser C, Gribonika I, Andersson M, Bostr, ö, m J, Alalam H, Harandi AM, Farewell A, L, ö, tvall J, Detoxified synthetic bacterial membrane vesicles as a vaccine platform against bacteria and SARS-CoV-2. J Nanobiotechnology, 21(1):156(2023) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: vaccine delivery, label-free proteomics, synthetic bacterial vesicles,outer membrane vesicles (OMV)

Carina Sihlbom
contact affiliation	Proteomics Core Facility, Sahlgrenska Akademy, University of Gothenburg, Sweden
contact email	Carina.sihlbom@gu.se
lab head
Evelin Berger
contact affiliation	Proteomics Core Facility, University of Gothenburg
contact email	evelin.berger@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD033690

PRIDE project URI

• PRIDE
  1. PXD033690
     1. Label: PRIDE project
     2. Name: Detoxified synthetic bacterial membrane vesicles as a platform of vaccines against bacteria and SARS-CoV-2