PXD033676

PXD033676 is an original dataset announced via ProteomeXchange.

Title	Isolation of a novel heterodimeric PSII complex involved in PSII repair
Description	The multi-subunit membrane protein complex Photosystem II (PSII) catalyzes the light driven oxidation of water and with this the initial step of photosynthetic electron transport in plants, algae, and cyanobacteria. Its biogenesis is coordinated by a network of auxiliary proteins that facilitate the stepwise assembly of individual subunits and cofactors, forming various intermediate complexes until fully functional mature PSII is present at the end of the process. In the current study, we purified PSII complexes from a mutant line of the thermophilic cyanobacterium Thermosynechococcus elongatus in which the extrinsic subunit PsbO, characteristic for active PSII, was fused with an N-terminal TwinStrep-Tag. Three distinct PSII complexes were separated by ion exchange chromatography after the initial affinity purification. Two complexes differ in their oligomeric state (monomeric and dimeric) but share the typical subunit composition of mature PSII. They are characterized by very high oxygen evolving activity of approx. 6,000 µmol O2· (mg Chl·h)-1. Analysis of the third (heterodimeric) PSII complex revealed lower oxygen evolving activity of approx. 3000 µmol O2· (mg Chl·h)-1 and a manganese content of 2.7 (± 0.2) per reaction center compared to 3.7 (± 0.2) of fully active PSII. Mass spectrometry and time-resolved fluorescence spectroscopy further indicated that PsbO is partially replaced by Psb27 in this PSII fraction, thus implying a role in repair of the complex.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:23:48.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Thermosynechococcus elongatus BP-1; NCBI TaxID: 197221;
ModificationList	acetylated residue; monohydroxylated residue; formylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 00:43:53	ID requested
1	2023-02-06 08:42:05	announced
⏵ 2	2023-11-14 08:23:49	announced	2023-11-14: Updated project metadata.

Lambertz J, Meier-Credo J, Kucher S, Bordignon E, Langer JD, Nowaczyk MM, Isolation of a novel heterodimeric PSII complex via strep-tagged PsbO. Biochim Biophys Acta Bioenerg, 1864(2):148953(2023) [pubmed]

submitter keyword: cyanobacteria, PsbO, Thermosynechococcus elongatus,Photosynthesis, Photosystem II

Julian Langer
contact affiliation	MPI of Biophysics, MPI for Brain Research
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033676
     1. Label: PRIDE project
     2. Name: Isolation of a novel heterodimeric PSII complex involved in PSII repair