PXD033674

PXD033674 is an original dataset announced via ProteomeXchange.

Title	Investigation upon the state of p53 as well as interaction partners, in so much as can be learned from using the multi-component v5-dTag system.
Description	Chromatin modifications provide additional context-dependence for DNA sequence-based gene regulation. Binding sites of the transcription factor (TF) and important tumour suppressor p53 are unusually diverse with regards to their chromatin accessibility and histone modifications, suggesting different modes of binding. Here, we show that the ability of p53 to open chromatin and activate its target genes is locally restricted by its cofactor Trim24. The histone-binding domains of Trim24 limits the role of p53 at closed chromatin but not at accessible chromatin where Trim24 is blocked by histone 3 methylation at lysine 4. In turn, p53 regulates gene expression as a function of the naïve chromatin state prior to activation. These findings establish a novel mode of gene regulation by p53 in closed chromatin and illustrate how histone modification sensing cofactors can bridge local chromatin state and TF potency.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:50.215.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Hess
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 00:35:23	ID requested
1	2023-05-05 02:35:47	announced
2	2023-07-03 03:48:51	announced	2023-07-03: Updated project metadata.
⏵ 3	2023-11-14 09:01:58	announced	2023-11-14: Updated project metadata.

Isbel L, Iskar M, Durdu S, Weiss J, Grand RS, Hietter-Pfeiffer E, Kozicka Z, Michael AK, Burger L, Thom, ä NH, Sch, ü, beler D, Readout of histone methylation by Trim24 locally restricts chromatin opening by p53. Nat Struct Mol Biol, 30(7):948-957(2023) [pubmed]

submitter keyword: Trim24, gene regulation, Chromatin,p53

Dirk Schuebeler
contact affiliation	Department of Gene Regulation Friedrich Miescher Institute for Biomedical Research
contact email	dirk.schubeler@fmi.ch
lab head
Daniel Hess
contact affiliation	Friedrich Miescher Institute for Biomedical Research
contact email	dhess@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD033674

PRIDE project URI

• PRIDE
  1. PXD033674
     1. Label: PRIDE project
     2. Name: Investigation upon the state of p53 as well as interaction partners, in so much as can be learned from using the multi-component v5-dTag system.