PXD033668

PXD033668 is an original dataset announced via ProteomeXchange.

Title	Top-Down proteomics and Bottom-Up terminomics for the Detection of Protein Termini in C. elegans
Description	Here we investigate how top-down proteomics (TDP) can be of value for the detection of protein termini. Either GELFrEE or solid-phase enrichment was conducted for pre-fraction. To verify the detected N-termini HUNTER (High-efficiency Undecanal-based N Termini EnRichment) was employed. Reductive dimethylation on intact protein level was additionally applied.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:23.210.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 00:33:33	ID requested
1	2022-09-26 04:23:58	announced
⏵ 2	2023-11-14 08:56:23	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Orbitrap Q Exactive Plus, GELFrEE, MSPathFinder, Top-Down Proteomic,C. Elegans, Orbitrap Fusion Lumos, Informed Proteomics, Bottom-Up Proteomics, ProSight

Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, Kiel, Germany (lab head)
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD033668

PRIDE project URI

• PRIDE
  1. PXD033668
     1. Label: PRIDE project
     2. Name: Top-Down proteomics and Bottom-Up terminomics for the Detection of Protein Termini in C. elegans