PXD033664

PXD033664 is an original dataset announced via ProteomeXchange.

Title	Integrating proteomic data with metabolic modelling provides insight into key pathways in Bordetella pertussis biofilms
Description	Pertussis, commonly known as whooping cough is a severe respiratory disease caused by the bacterium, Bordetella pertussis. Despite widespread vaccination, pertussis resurgence has been observed globally. The development of the current acellular vaccine (ACV) has been based on planktonic studies. However, recent studies have shown that B. pertussis readily forms biofilms. A better understanding of B. pertussis biofilms is important for developing novel vaccines that can target all aspects of B. pertussis infection. This study compared the proteomic expression of biofilm and planktonic B. pertussis cells to identify key changes between the conditions. Major differences were identified in virulence factors including an upregulation of toxins (adenylate cyclase toxin and dermonecrotic toxin) and strong downregulation of pertactin and type III secretion system proteins in biofilm cells. To further dissect metabolic pathways that are altered during the biofilm lifestyle, the proteomic data was then incorporated into a genome scale metabolic model using the integrated metabolic analysis tool (iMAT). The analysis revealed that planktonic cells utilised the glyoxylate shunt while biofilm cells completed the full tricarboxylic acid cycle. Differences in processing aspartate, arginine and alanine were identified as well as unique export of valine out of biofilm cells which may have a role in inter-bacterial communication and regulation. Finally, increased polyhydroxybutyrate accumulation and superoxide dismutase activity in biofilm cells may contribute to increased persistence during infection. Taken together, this study modelled major proteomic and metabolic changes that occur in biofilm cells which helps lay the groundwork for further understanding B. pertussis pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:04:26.781.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033664
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hiroki Suyama
SpeciesList	scientific name: Bordetella pertussis; NCBI TaxID: 520;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 00:24:59	ID requested
1	2023-08-07 00:26:39	announced
⏵ 2	2023-11-14 07:04:27	announced	2023-11-14: Updated project metadata.

10.6019/PXD033664;

submitter keyword: LC-MSMS, LFQ,Bordetella pertussis, biofilm

Ruiting Lan
contact affiliation	School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia (lab head)
contact email	r.lan@unsw.edu.au
lab head
Hiroki Suyama
contact affiliation	University of New South Wales, Sydney
contact email	h.suyama@unsw.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033664
     1. Label: PRIDE project
     2. Name: Integrating proteomic data with metabolic modelling provides insight into key pathways in Bordetella pertussis biofilms