PXD033659

PXD033659 is an original dataset announced via ProteomeXchange.

Title	The identification of onchocerciasis biomarker candidates directly in human serum and urine
Description	Onchocerca volvulus, the causative agent of onchocerciasis, infects over 20 million people and can cause severe dermatitis and ocular conditions including blindness. Current mass drug administration treatments do not kill female O. volvulus worms, and common diagnostic tests cannot reliably assess the viability of adult worms. This lack of sensitivity for active infections presents an urgent need for better diagnostic tests to monitor the efficacy of new treatments and ongoing mass drug administration. Serum samples from individuals infected with O. volvulus (n = 8), and from uninfected individuals (n = 7) were examined by deep scale proteomics, including the use of a timsTOF Pro mass spectrometer. Data were interrogated for O. volvulus proteins present in infected but not uninfected samples. Among all detected proteins, 19 high-priority candidate biomarkers were identified in the serum of 3 or more O. volvulus-infected samples (not present in uninfected) that were supported by two or more unique peptides. Comprehensive functional annotation, RNA-seq based transcriptional profiling and taxonomic conservation/diversification characterized the detected proteins in more detail. 15 peptides from 11 of the top 19 proteins were validated by parallel MS/MS (Orbitrap) with isotope-labeled synthetic peptides. One candidate was detected with eight unique peptides in five different serum samples. Additional MS/MS samples have shown that peptides from 4 of the top 6 candidate biomarkers were also detected in urine samples from onchocerciasis patients. We are now working to develop practical assays for the most promising candidates that may be useful for diagnosis of active infections and for monitoring efficacy of new treatments in clinical trials.
HostingRepository	iProX
AnnounceDate	2022-11-22
AnnouncementXML	Submission_2023-01-28_19:51:24.970.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Martin
SpeciesList	scientific name: Onchocerca volvulus; NCBI TaxID: 6282; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 19:18:31	ID requested
1	2022-11-22 00:29:22	announced
⏵ 2	2023-01-28 19:51:25	announced	2023-01-29: Update publication information.

Rosa BA, Curtis K, Erdmann Gilmore P, Martin J, Zhang Q, Sprung R, Weil GJ, Townsend RR, Fischer PU, Mitreva M, Direct Proteomic Detection and Prioritization of 19 Onchocerciasis Biomarker Candidates in Humans. Mol Cell Proteomics, 22(1):100454(2023) [pubmed]

submitter keyword: Onchocerciasis, biomarker, LC-MSMS, serum, urine, Onchocerca volvulus

Peter U Fisher
contact affiliation	Washington University in St. Louis School of Medicine; Department of Medicine, Division of Infectious Diseases
contact email	pufisher@wustl.edu
lab head
John Martin
contact affiliation	Washington University in St. Louis School of Medicine; Department of Medicine, Division of Infectious Diseases
contact email	jmartin@wustl.edu
dataset submitter

iProX dataset URI