PXD033658

PXD033658 is an original dataset announced via ProteomeXchange.

Title	Lockd promotes myoblast proliferation and skeletal muscle regeneration through binding with DHX36 to facilitate unwinding of 5’UTR rG4 and translation of Anp32e mRNA
Description	Adult muscle stem cells, also known as satellite cells (SCs) play pivotal roles in injury induced muscle regeneration and our knowledge of long non-coding RNA (lncRNA) functions in SCs remains limited. Here we identify a lncRNA, Lockd which is induced in activated SCs upon acute muscle injury. We demonstrate that Lockd promotes SC proliferation; deletion of Lockd leads to cell cycle arrest at G1/S phase. Consistently, in vivo repression of Lockd in mouse muscles hinders muscle regeneration process. Mechanistically, we uncover that Lockd lncRNA molecule directly interacts with an RNA helicase DHX36; structural probing further shows that the 5’end of the Lockd possesses the strongest binding activity with DHX36 protein. Furthermore, we demonstrate that Lockd stabilizes the interaction between DHX36 and EIF3B proteins; and synergistically this complex unwinds the RNA G-quadruplex (rG4) structure formed at the 5’UTR of Anp32e mRNA and promotes the translation of ANP32E protein which is required for myoblast cell proliferation. Altogether, our findings thus identify a regulatory circuitry consisting of Lockd/DHX36/Anp32e that functions to promote myoblast proliferation, thus enabling the acute injury induced muscle regeneration to progress.
HostingRepository	iProX
AnnounceDate	2022-05-24
AnnouncementXML	Submission_2023-12-25_20:07:11.871.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaona Chen
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 19:09:05	ID requested
1	2022-05-23 20:21:51	announced
⏵ 2	2023-12-25 20:07:12	announced	2023-12-26: Update information.

Chen X, Xue G, Zhao J, Zhang Y, Zhang S, Wang W, Li Y, Yuan J, He L, Chan CY, Liu Y, Chen W, Zhao Y, Hu P, Sun H, Kwok CK, Wang H, Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation. Cell Rep, 39(10):110927(2022) [pubmed]

submitter keyword: Lockd, RNA G-quadruplexes, DHX36, satellite cells, muscle regeneration, translational regulation

Huating Wang
contact affiliation	The Chinese University of Hong Kong
contact email	huating.wang@cuhk.edu.hk
lab head
Xiaona Chen
contact affiliation	The Chinese University of Hong Kong
contact email	xiaonachan@gmail.com
dataset submitter

iProX dataset URI