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PXD033650

PXD033650 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDe novo mapping of the apicomplexan Ca2+-responsive proteome: concentration range thermal profiling experiment 2
DescriptionApicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition between the replicative and lytic phases of the infectious cycle. Despite the presence of conserved Ca2+-responsive proteins, little is known about how specific signaling elements interact to impact pathogenesis. We mapped the Ca2+-responsive proteome of the model apicomplexan T. gondii via time-resolved phosphoproteomics and thermal proteome profiling. The waves of phosphoregulation following PKG activation and stimulated Ca2+ release corroborate known physiological changes but identify specific molecular components in these pathways. Thermal profiling of parasite extracts identified many expected Ca2+-responsive proteins, such as parasite Ca2+-dependent protein kinases. Our approach also identified numerous Ca2+-responsive proteins that are not predicted to bind Ca2+, yet are critical components of the parasite signaling network. We characterized protein phosphatase 1 (PP1) as a Ca2+-responsive enzyme that relocalized to the parasite apex upon Ca2+ store release. Conditional depletion of PP1 revealed that the phosphatase regulates Ca2+ uptake to promote parasite motility. PP1 may thus be partly responsible for Ca2+-regulated serine/threonine phosphatase activity in apicomplexan parasites.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:46:15.799.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD033650
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlice Herneisen
SpeciesList scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; 6x(13)C; methylthiolated residue; 6x(13)C; phosphorylated residue; monohydroxylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-04 15:10:52ID requested
12024-10-07 13:46:16announced
Publication List
10.7554/elife.80336;
Herneisen AL, Li ZH, Chan AW, Moreno SNJ, Lourido S, -responsive pathways. Elife, 11():(2022) [pubmed]
10.6019/PXD033650;
Keyword List
submitter keyword: Toxoplasma, thermal proteome profiling, proteomics, parasite, calcium signaling, CETSA, TPP, apicomplexan, systems biology
Contact List
Sebastian Lourido
contact affiliationAssociate Professor, Department of Biology, Massachusetts Institute of Technology and Whitehead Institute of Biomedical Research
contact emaillourido@wi.mit.edu
lab head
Alice Herneisen
contact affiliationWhitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology
contact emailalice@wi.mit.edu
dataset submitter
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