PXD033645

PXD033645 is an original dataset announced via ProteomeXchange.

Title	O-Glycoproteomic Analysis of Engineered Heavily-Glycosylated Fusion Proteins using NanoHILIC-MS
Description	In this study, we identified and quantified the relative glycan distribution on UTI-Fc using nanoHILIC-MS for comprehensive mapping O-glycopeptides in UTI-Fc fusion protein. We quantified O-glycosylated peptides in the Fab domain and describe in detail for the first-time unexpected O-glycosylation at the linker and Fc region in UTI-Fc. These results highlight the improved performance of nanoHILIC-MS for characterization of biotherapeutic protein glycosylation in comparison to RP nanoLC-MS methods.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:37.814.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gustavo Cavallero
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 14:44:13	ID requested
1	2023-03-11 04:56:30	announced
⏵ 2	2023-11-14 08:42:42	announced	2023-11-14: Updated project metadata.

Cavallero GJ, Wang Y, Nwosu C, Gu S, Meiyappan M, Zaia J, O-Glycoproteomic analysis of engineered heavily glycosylated fusion proteins using nanoHILIC-MS. Anal Bioanal Chem, 414(27):7855-7863(2022) [pubmed]

submitter keyword: fusion proteins, glycoproteomics

Gustavo Cavallero
contact affiliation	Zaia Lab, Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, Massachusetts 02118, United States
contact email	gcaval@bu.edu
lab head
Gustavo Cavallero
contact affiliation	Boston University
contact email	gcaval@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033645

PRIDE project URI

• PRIDE
  1. PXD033645
     1. Label: PRIDE project
     2. Name: O-Glycoproteomic Analysis of Engineered Heavily-Glycosylated Fusion Proteins using NanoHILIC-MS