PXD033623

PXD033623 is an original dataset announced via ProteomeXchange.

Title	Protein turnover analysis in Salmonella Typhimurium during infection by dynamic SILAC, Topograph and quantitative proteomics
Description	Protein turnover affects protein abundance and phenotypes. Comprehensive investigation of protein turnover dynamics has the potential to provide substantial information about gene expression. Here we report a large-scale protein turnover study in Salmonella Typhimurium during infection by quantitative proteomics. Murine macrophage-like RAW 264.7 cells were infected with SILAC labeled Salmonella. Bacterial cells were extracted after 0, 30, 60, 120 and 240 min. Mass spectrometry analyses yielded information about Salmonella protein turnover dynamics and a software program named Topograph was used for the calculation of protein half lives. The half lives of 311 proteins from intracellular Salmonella were obtained. For bacteria cultured in control medium (DMEM), the half lives for 870 proteins were obtained. The calculated median of protein half lives was 69.13 min and 99.30 min for the infection group and the DMEM group respectively, indicating an elevated protein turnover at the initial stage of infection. Gene ontology analyses revealed that a number of protein functional groups were significantly regulated by infection, including proteins involved in ribosome, periplasmic space, cellular amino acid metabolic process, ion binding and catalytic activity. The half lives of proteins involving in purine metabolism pathway were found to be significantly shortened during infection.
HostingRepository	PRIDE
AnnounceDate	2022-05-17
AnnouncementXML	Submission_2022-05-17_10:55:53.457.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhe Wang
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Typhimurium; NCBI TaxID: 90371;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 13:52:32	ID requested
⏵ 1	2022-05-17 10:55:54	announced

Wang Z, Han QQ, Zhou MT, Chen X, Guo L, Protein turnover analysis in Salmonella Typhimurium during infection by dynamic SILAC, Topograph, and quantitative proteomics. J Basic Microbiol, 56(7):801-11(2016) [pubmed]

submitter keyword: Salmonella Typhimurium, proteomics, dynamic SILAC, protein turnover

Lin Guo
contact affiliation	State Key Laboratory of Virology, College of Life Sciences, Wuhan University
contact email	guol@whu.edu.cn
lab head
Zhe Wang
contact affiliation	Wuhan University
contact email	wang_zhe@whu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033623
     1. Label: PRIDE project
     2. Name: Protein turnover analysis in Salmonella Typhimurium during infection by dynamic SILAC, Topograph and quantitative proteomics