PXD033620

PXD033620 is an original dataset announced via ProteomeXchange.

Title	Comparative genomics and interactomics of polyadenylation factors for the prediction of new parasite targets: Entamoeba histolytica as a working model
Description	E. histolytica trophozoites (3x105) harvested in exponential growth phase were transferred to 25 cm² cell culture flasks and grown at 37°C for 24 h. Then, parasites were exposed to irradiation in a 254 nm UV-transilluminator (BioDoc-It Imaging System (UVP)) for 30 min at 4°C (Valdés et al., 2014) and cytoplasmic (CE) and nuclear (NE) extracts were obtained using the NE-PER Kit (Thermo Scientific™) according to manufacturer instructions. Immunoprecipitation (IP) assays were performed using Dynabeads protein A/G (Invitrogen) following manufacturer recommendations. Briefly, Dynabead magnetic beads (25 µl) were coupled with 10 µg of anti-HsCFIm25 polyclonal antibody (GeneTex, GTX115535) and anti-EhCFIm25 polyclonal antibody, previously produced and validated by our group (Pezet-Valdez et al., 2013) for 30 min at room temperature. Then, beads-antibody complex was incubated with NE (100 µg) for 2 h at 37°C followed by three washes with wash buffer (20 mM Tris, pH 7.5; 0.5M NaCl and 0.05% Tween 20). Finally, magnetic bead- antibodies-proteins complex was resuspended in elution buffer (0.1 M glycine, pH 2.0) for 2 minutes at room temperature to dissociate the complex; the tube was placed on the magnet and the supernatant containing eluted antibody and bound proteins was collected.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:45.013.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rodolfo G Avila-Bonilla
SpeciesList	scientific name: Entamoeba histolytica HM-1:IMSS-A; NCBI TaxID: 885318;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 10:15:18	ID requested
1	2023-03-11 04:11:24	announced
⏵ 2	2023-11-14 08:42:50	announced	2023-11-14: Updated project metadata.

Avila-Bonilla RG, Velazquez-Guzman JA, Reyes-Zepeda EI, Gutierrez-Avila JL, Reyes-L, ó, pez CA, Cisneros-Sarabia A, Saavedra E, Lop, é, z-Sandoval A, Ram, í, rez-Moreno E, L, ó, pez-Camarillo C, Marchat LA, Comparative genomics and interactomics of polyadenylation factors for the prediction of new parasite targets: Entamoeba histolytica as a working model. Biosci Rep, 43(2):(2023) [pubmed]

submitter keyword: mRNA processing

protein-protein interactions

Entamoeba histolytica

Laurence A. Marchat
contact affiliation	Instituto Politécnico Nacional
contact email	lmarchat@gmail.com
lab head
Rodolfo G Avila-Bonilla
contact affiliation	Instituto Politécnico Nacional
contact email	avilab.r.g@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033620

PRIDE project URI

• PRIDE
  1. PXD033620
     1. Label: PRIDE project
     2. Name: Comparative genomics and interactomics of polyadenylation factors for the prediction of new parasite targets: Entamoeba histolytica as a working model