PXD033611

PXD033611 is an original dataset announced via ProteomeXchange.

Title	Global analysis of neuropeptides in cestodes identifies Attachin, a SIFamide homolog, as a stimulant of parasite motility and attachment
Description	Many anthelmintics target the neuromuscular system, in particular by interfering with signalling mediated by classical neurotransmitters. Although peptidergic signalling has been proposed as a target of novel anthelmintics, current knowledge of the neuropeptide complement of many helminths is still limited, especially for parasitic flatworms (cestodes, trematodes and monogeneans). In this work, we have characterised the neuropeptide complement of the model cestode Hymenolepis microstoma. Peptidomic characterization of adults of H. microstoma validated many of the neuropeptide precursor (npp) genes previously predicted in silico, and identified novel neuropeptides that are conserved in parasitic flatworms. Most neuropeptides from parasitic flatworms lack significant similarity to those from other animals, confirming the uniqueness of their peptidergic signalling. Analysis of gene expression of ten npp genes by in situ hybridization confirmed that all of them are expressed in the nervous system and identified cryptic features, including the first evidence of dorsoventral asymmetry, as well as a new population of peripheral neurosecretory cells that appears to be conserved in the trematode Schistosoma mansoni. Finally, we characterised in greater detail Attachin, an experimentally validated SIFamide homolog. Although its expression is largely restricted to the longitudinal nerve cords and cerebral commissure in H. microstoma, it shows widespread expression in the larval nervous system of Echinococcus multilocularis and Mesocestoides corti. Exogenous addition of a peptide corresponding to the highly conserved C-terminus of Attachin stimulated motility and attachment of M. corti larvae. Altogether, this work provides a robust experimental foothold for the characterization of peptidergic signalling in parasitic flatworms.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:50.865.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033611
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sven Van Bael
SpeciesList	scientific name: Hymenolepis microstoma; NCBI TaxID: 85433;
ModificationList	amidated residue; 2-pyrrolidone-5-carboxylic acid (Glu); half cystine; 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 10:05:21	ID requested
1	2022-06-13 04:14:44	announced
⏵ 2	2023-11-14 08:56:51	announced	2023-11-14: Updated project metadata.

10.6019/PXD033611;

submitter keyword: peptidomics, sucker, motility, peptidergic, SIFamide,tapeworm

Uriel Koziol
contact affiliation	Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
contact email	ukoziol@fcien.edu.uy
lab head
Sven Van Bael
contact affiliation	KU Leuven
contact email	sven.vanbael@kuleuven.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033611
     1. Label: PRIDE project
     2. Name: Global analysis of neuropeptides in cestodes identifies Attachin, a SIFamide homolog, as a stimulant of parasite motility and attachment