PXD033595

PXD033595 is an original dataset announced via ProteomeXchange.

Title	Proteomics, phosphoproteomics and miRNA analysis of circulating extracellular vesicles through automated and high-throughput isolation
Description	Here, we introduce a magnetic bead-based EV enrichment approach (EVrich) for automated and high-throughput processing of urine samples. Parallel enrichments can be performed in 96-well plates for downstream cargo analysis, including EV characterization, miRNA, proteomics and phosphoproteomics analysis. We applied the instrument to a cohort of clinical urine samples to achieve reproducible identification of over 47,562 unique EV peptides and 4,327 EV proteins in each one-ml urine sample. Quantitative phosphoproteomicsrevealed 186 unique phosphopeptides corresponding to 77 proteins that were significantly elevated in prostate cancer patients.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:10:01.277.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hao Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 09:16:17	ID requested
⏵ 1	2022-08-11 23:10:02	announced

Zhang H, Cai YH, Ding Y, Zhang G, Liu Y, Sun J, Yang Y, Zhan Z, Iliuk A, Gu Z, Gu Y, Tao WA, Proteomics, Phosphoproteomics and Mirna Analysis of Circulating Extracellular Vesicles through Automated and High-Throughput Isolation. Cells, 11(13):(2022) [pubmed]

submitter keyword: Extracellular vesicles, miRNA, phosphoproteomics, mass spectrometry, prostate cancer

W. Andy Tao
contact affiliation	State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing 210096, China
contact email	zhanghaohao@seu.edu.cn
lab head
Hao Zhang
contact affiliation	Southeast University
contact email	zhanghaohao@seu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033595

PRIDE project URI

• PRIDE
  1. PXD033595
     1. Label: PRIDE project
     2. Name: Proteomics, phosphoproteomics and miRNA analysis of circulating extracellular vesicles through automated and high-throughput isolation