PXD033577

PXD033577 is an original dataset announced via ProteomeXchange.

Title	Total proteome analysis of engineered/adapted E.coli strains with glucose/xylose co-utilization ability
Description	This project was about developing a bacterial strain that could consume a mixture of glucose and xylose simultaneously with higher ethanol productivity. In this study, an ethanologenic strain (SSK42) was made deficient in Carbon Catabolite Repression (CCR) by deleting the ptsG gene encoding EIIBCGlc component of PTS transport system. This strain (SCD00) was then evolved for several generations on xylose containing minimal media. A strain (SCD78) was finally obtained that, unlike its parent strain could consume glucose and xylose simultaneously. Then we performed proteomics analysis of evolved and un-evolved strain. The strain SSK42 was also considered for proteome analysis as a reference for analysis. The starting strain – SSK42, is the derivative of E.coli B.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:18:28.379.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033577
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chandra Dev
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 08:16:03	ID requested
1	2022-10-14 21:27:31	announced
⏵ 2	2023-11-14 07:18:29	announced	2023-11-14: Updated project metadata.

10.6019/PXD033577;

Dev C, Jilani SB, Yazdani SS, Adaptation on xylose improves glucose-xylose co-utilization and ethanol production in a carbon catabolite repression (CCR) compromised ethanologenic strain. Microb Cell Fact, 21(1):154(2022) [pubmed]

submitter keyword: Sugar mixture, Orbitrap lumos,E.coli B, Co-utilization

Syed Shams Yazdani
contact affiliation	Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
contact email	shams@icgeb.res.in
lab head
Chandra Dev
contact affiliation	International Centre for Genetic Engineering and Biotechnology
contact email	chandradev@icgeb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD033577

PRIDE project URI

• PRIDE
  1. PXD033577
     1. Label: PRIDE project
     2. Name: Total proteome analysis of engineered/adapted E.coli strains with glucose/xylose co-utilization ability