PXD033568

PXD033568 is an original dataset announced via ProteomeXchange.

Title	Establishment of a landscape of UPL5-ubiquitinated on multiple subcellular components of leaf senescence cell in Arabidopsis
Description	Catabolism of macromolecules is a major event in senescent cells, especially involving proteolysis of organelles and abnormally aggregated proteins, the circulation of nutrients, and precise control of intracellular environmental balance. Proteasomes are distributed in the nucleus and cytoplasm; however, proteasome in organelles is limited. In this study, multi-omics proteomic analyses of ubiquitinated proteins enriched by using antibody against “di-Gly-Lys” via a free labeling was used to investigate the global changes of protein levels and ubiquitination modification levels of upl5 mutant relative to wildtype plant; subcellular localization analysis of UPL5 was found to be located in the nucleus, cytoplasm and plastid within the cell; and the direct lysine site pattern of UPL5 were screened by the H89R substitution in the tagged ubiquitinated assay. It suggests that UPL5 acting as a candidate of organelle E3 ligase either in the nucleus or cytoplasm or plastid, modifies numerous targets related to nuclear transcription and plastid photosynthesis involving in Ca2+ and hormone signaling pathway in plant senescence and in response to (a)biotic stress protection.
HostingRepository	iProX
AnnounceDate	2022-04-30
AnnouncementXML	Submission_2023-05-06_00:10:25.330.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Lan
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	ubiquitinylated lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-30 06:40:33	ID requested
1	2023-05-04 19:03:13	announced
⏵ 2	2023-05-06 00:10:27	announced	2023-05-06: Update publication information.

Lan W, Zheng S, Yang P, Qiu Y, Xu Y, Miao Y, . Int J Mol Sci, 23(10):(2022) [pubmed]

Lan W, Ma W, Zheng S, Yang P, Qiu Y, Lin W, Ren Y, Miao Y, -induced leaf senescence. iScience, 26(3):106216(2023) [pubmed]

submitter keyword: Integrative analysis, UPL5, subcellular localization, ubiquitination, leaf senescence

Ying Miao
contact affiliation	Fujian Provincial Key Laboratory of Functional Biology, Fujian Agriculture and Forestry University
contact email	ymiao@fafu.edu.cn
lab head
Wei Lan
contact affiliation	Fujian Agriculture and Forestry University
contact email	1150538001@fafu.edu.cn
dataset submitter

iProX dataset URI