PXD033555

PXD033555 is an original dataset announced via ProteomeXchange.

Title	Analysis of citrullination of extracellular matrix proteins in spheroid cultures of fibroblasts and cancer cells
Description	Eight different human cancer cell lines were cocultured with human cancer associated fibroblasts (CAF) as spheroid cultures. In another setup UT-SCC-7 cutaneous squamous cell carcinoma cells, together with human skin fibroblasts (SFB) and UT-SCC-2 cells together with gingival fibroblasts (GFB) were cultured as spheroid cocultures. These spheroids were treated with PAD4 or with citrullination buffer only. All spheroids were hypotonically lysed, and the remaining insoluble material was digested to peptides and analysed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:25.052.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033555
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pekka Rappu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 02:40:35	ID requested
1	2022-11-21 03:26:47	announced
⏵ 2	2023-11-14 08:56:25	announced	2023-11-14: Updated project metadata.

10.6019/PXD033555;

submitter keyword: Citrullination, cancer, inflammation, spheroid

Jyrki Heino
contact affiliation	Department of Life Technologies, University of Turku, Turku, Finland
contact email	jyrki.heino@utu.fi
lab head
Pekka Rappu
contact affiliation	University of Turku
contact email	pekka.rappu@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033555
     1. Label: PRIDE project
     2. Name: Analysis of citrullination of extracellular matrix proteins in spheroid cultures of fibroblasts and cancer cells