PXD033537

PXD033537 is an original dataset announced via ProteomeXchange.

Title	Venomics of the Central-European Myrmicine Ants Myrmica rubra and Myrmica ruginodis
Description	Animal venoms are a rich source of novel biomolecules with tremendous potential in medicine and agriculture. Ants represent one of the most species-rich lineages of venomous animals. However, only a fraction of their biodiversity has been studied so far. Here, we investigated the venom compositions from Myrmica rubra and Myrmica ruginodis, two members of the Myrmicinae subfamily of ants. We applied a proteo-transcriptomics based venomics workflow. Our analysis revealed that venoms of both species are composed of several protein classes, such as venom serine protease, cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 proteins (CAP), Kunitz-type serine protease inhibitors or venom acid phosphatase. Several protein classes identified are known venom allergens, and for the first time we detected phospholipase A1 in the venom of M. ruginodis. We also identified two novel toxins of the epidermal growth factor (EGF) family in its venom proteome and an array of additional EGF-like toxins in venom gland transcriptomes of both species. They display similarity to known toxins from the related myrmecine Manica rubida and the Australian red bulldog ant Myrmecia gullosa of the Myrmeciinae subfamily and may serve as nociceptive weapons in defensive scenarios. Our work suggests that the venoms of M. rubra and M. ruginodis contain many high molecular proteins and enzymes with putatively cell damaging functions. Nevertheless, the presence of EGF-like toxins underpins that myrmicine ants also recruited smaller peptide components into their venom arsenal. Although little is known about the bioactivity and function of these EGF-like toxins, their presence in Myrmecinae and Myrmeciinae suggests that they play an important role for the venom systems of Formicoidea. Our work adds to the emerging picture of ant venoms as a source for novel biomolecules. This underlines the importance to incorporate such taxa in future venom bioprospecting programs.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_10:30:48.381.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033537
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Günter Lochnit
SpeciesList	scientific name: Myrmica rubra; NCBI TaxID: 106198; scientific name: Myrmica ruginodis; NCBI TaxID: 34708;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 01:44:17	ID requested
⏵ 1	2022-06-09 10:30:49	announced

Hurka S, Brinkrolf K, Ö, zbek R, F, ö, rster F, Billion A, Heep J, Timm T, Lochnit G, Vilcinskas A, L, ü, ddecke T, . Toxins (Basel), 14(5):(2022) [pubmed]

submitter keyword: Venomics, Proteomics, Ants, insect venom, proteo-transcriptomics

Lochnit, Guenter
contact affiliation	Institute of Biochemistry, Faculty of Medicine, University Giessen, Friedrichstrasse 24, 35392 Giessen, Germany
contact email	guenter.lochnit@biochemie.med.uni-giessen.de
lab head
Günter Lochnit
contact affiliation	Protein Analytics, Institute of Biiochemistry, Faculty of Medicine, University Giessen, Friedrichstrasse 24, 35392 Giessen
contact email	guenter.lochnit@biochemie.med.uni-giessen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD033537

PRIDE project URI

• PRIDE
  1. PXD033537
     1. Label: PRIDE project
     2. Name: Venomics of the Central-European Myrmicine Ants Myrmica rubra and Myrmica ruginodis