PXD033534

PXD033534 is an original dataset announced via ProteomeXchange.

Title	Dexamethasone is a regulator of human testicular peritubular cells / Proteome 72 h
Description	The functions of human testicular peritubular cells (HTPCs), which form a small compartment located between the seminiferous epithelium and the interstitials areas of the testis, go beyond intratesticular sperm transport and include immunological roles. The expression of the glucocorticoid receptor (GR/NR3C1) by these cells indicates that they are a target of glucocorticoids (GCs), yet detailed insights into consequences of GC actions are unknown. To address this point, we studied cultured HTPCs, which serve as a window into the human testis. We used a cytokine profiler assay to screen for changes in secreted cytokines upon dexamethasone (Dex) treatment and employed qPCR and ELISA studies to verify the results. Dex, used in a therapeutic concentration, decreased proinflammatory factors, including IL6, IL8, MCP1 and CXCL1 within 24 - 48 h. An siRNA-mediated knockdown of GR reduced these actions. Exposure to Dex resulted in reduced GR levels, implying that exposure to Dex could also reduce anti-inflammatory actions of Dex. To evaluate the influence of Dex on HTPCs further, we performed a proteomic analysis of cellular components and secreted proteins. Strong responses were detectable after 24 h (31 significantly altered cellular proteins) and more pronounced ones after 72 h (30 less abundant and 42 more abundant cellular proteins). Dex also altered the composition of the secretome (33 proteins decreased, 13 increased) after 72 h. Among the regulated proteins were ECM- and basement membrane components (e.g. FBLN2, COL1A2, COL3A1), as well as PTX3 and StAR. These results reveal that Dex via GR exerts profound actions in HTPCs. If transferrable to the human testis, changes specifically in ECM and the immunological state of the testis may result in men upon treatment with Dex for medical reasons. -Proteome Data Subset 72 h-
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:01.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Youli Konstantinovitch Stepanov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 01:43:21	ID requested
1	2023-03-11 05:21:09	announced
⏵ 2	2023-11-14 08:43:09	announced	2023-11-14: Updated project metadata.

Stepanov YK, Speidel JD, Herrmann C, Schmid N, Behr R, K, ö, hn FM, St, ö, ckl JB, Pickl U, Trottmann M, Fr, ö, hlich T, Mayerhofer A, Welter H, Profound Effects of Dexamethasone on the Immunological State, Synthesis and Secretion Capacity of Human Testicular Peritubular Cells. Cells, 11(19):(2022) [pubmed]

submitter keyword: Human male fertility

human testis

dexamethasone

cytokines

inflammation

proteomics

Dr. Thomas Fröhlich
contact affiliation	Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU München, München, Germany, Feodor-Lynen-Straße 25, 81377 München
contact email	frohlich@genzentrum.lmu.de
lab head
Youli Konstantinovitch Stepanov
contact affiliation	LMU München Genzentrum-LAFUGA
contact email	Stepanov@genzentrum.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033534

PRIDE project URI

• PRIDE
  1. PXD033534
     1. Label: PRIDE project
     2. Name: Dexamethasone is a regulator of human testicular peritubular cells / Proteome 72 h