PXD033533

PXD033533 is an original dataset announced via ProteomeXchange.

Title	Neuromelanin granules of the substantia nigra: proteomic profile provides links to tyrosine hydroxylase, stress granules and lysosomes
Description	Neuromelanin is a black-brownish pigment, present in so-called neuromelanin granules (NMGs) in the cell bodies of dopaminergic neurons in the substantia nigra (SN) pars compacta. These neurons are lost in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Although it is known that lipids, proteins, and environmental toxins accumulate in NMGs, the function of NMGs has not yet been finally clarified as well as their origin and the synthesis of neuromelanin. We therefore isolated NMGs and surrounding SN tissue from control patients by laser microdissection and analyzed the proteomic profile by tandem mass spectrometry. With our improved workflow, we were able to 1) strengthen the regularly reported link between NMGs and lysosomes, 2) detect tyrosine hydroxylase to be highly abundant in NMGs, which may be related to neuromelanin synthesis and 3) indicate a yet undescribed link between stress granules (SGs) and NMGs. Based on our findings, we cautiously hypothesize, that SGs may be the origin of NMGs or form in close proximity to them, potentially due to the oxidative stress caused by neuromelanin-bound metals.
HostingRepository	PRIDE
AnnounceDate	2022-08-29
AnnouncementXML	Submission_2022-08-29_06:35:15.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maximilian Wulf
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 01:38:59	ID requested
⏵ 1	2022-08-29 06:35:15	announced

Wulf M, Barkovits K, Schork K, Eisenacher M, Riederer P, Gerlach M, Eggers B, Marcus K, Neuromelanin granules of the substantia nigra: proteomic profile provides links to tyrosine hydroxylase, stress granules and lysosomes. J Neural Transm (Vienna), 129(10):1257-1270(2022) [pubmed]

submitter keyword: Neuromelanin granules, substantia nigra pars compacta, laser microdissection, mass spectrometry, stress granules, neuromelanin synthesis

Katrin Marcus
contact affiliation	Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, Bochum, Germany
contact email	katrin.marcus@rub.de
lab head
Maximilian Wulf
contact affiliation	Medizinisches Proteom-Center, Ruhr-University Bochum
contact email	Maximilian.Wulf@ruhr-uni-bochum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033533
     1. Label: PRIDE project
     2. Name: Neuromelanin granules of the substantia nigra: proteomic profile provides links to tyrosine hydroxylase, stress granules and lysosomes