PXD033510

PXD033510 is an original dataset announced via ProteomeXchange.

Title	Targeting PCNA in a subgroup of Multiple Myeloma cells inhibits the glycolysis and pentose phosphate pathway
Description	PCNA is an essential protein in all cells with multiple well-known function in DNA replication and DNA repair. Recently, PCNA was shown to be involved in regulation of cellular signalling and central carbon metabolism via stabilizing the metabolic enzymes ENO1 and 6PGD. Furthermore, ATX-101, a PCNA targeting peptide currently in Phase II, was shown to reduce metabolite pools in the glycolysis and the pentose phosphate pathway (PPP) in multiple haematological cells. Here integrated analysis of signalome and metabolome data from multiple myeloma (MM) cells reveals large effects of ATX-101 on signalling, metabolism and red-ox regulation. The sensitivity to ATX-101 varied between the eight MM cell lines studied and a metabolic shift was detected in the sensitive cell lines. These cell lines were characterized by low endogenous levels of the redox metabolites NAD+, NADH and GSH. Interestingly, 11 proteins were pulled down from untreated cells from the sensitive cell lines while not from 9 other cancer cell lines or primary monocytes from three donors. These proteins could be potential biomarkers for MM cells with low red-ox capacity and hypersensitivity for inhibitors of glycolysis or the PPP.
HostingRepository	PRIDE
AnnounceDate	2024-11-25
AnnouncementXML	Submission_2024-11-24_23:33:09.103.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 01:13:13	ID requested
⏵ 1	2024-11-24 23:33:09	announced

Dataset with its publication pending

submitter keyword: Targeting, pentose phosphate pathway, TK, Multiple Myeloma, cells, glycolysis, MiB assay, PCNA

Marit Otterlei
contact affiliation	Professor, Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim 7030, Norway
contact email	marit.otterlei@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033510
     1. Label: PRIDE project
     2. Name: Targeting PCNA in a subgroup of Multiple Myeloma cells inhibits the glycolysis and pentose phosphate pathway