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PXD033506 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGLH/VASA helicases promote germ granule formation to ensure the fidelity of piRNA-mediated transcriptome surveillance
DescriptionThe ability to distinguish non-self from self is the key characteristic for any defense system. piRNAs function as guardians of the genome by silencing non-self nucleic acids and transposable elements in animals. Many piRNA factors are enriched in perinuclear germ granules, but whether their localization is required for piRNA biogenesis or function is not known. Here we show that GLH/VASA helicase mutants exhibit defects in forming perinuclear condensates containing PIWI and other small RNA cofactors. These mutant animals produce largely normal levels of piRNA but are defective in triggering piRNA silencing. Strikingly, while many piRNA targets are activated in GLH mutants, we observed that hundreds of endogenous genes are aberrantly silenced by piRNAs. This defect in self versus non-self recognition was also observed in other mutants where perinuclear P granules are disrupted. Together, our results argue that perinuclear germ granules function critically to promote the fidelity of piRNA-based transcriptome surveillance in C. elegans and preserve self versus non-self distinction.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDonglei Zhang
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-28 01:07:22ID requested
12022-08-04 22:33:31announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: VASA, GLH-1
Contact List
Donglei Zhang
contact affiliationHuazhong University of Science and Technology, Wuhan, China
contact emailzhang_donglei@hust.edu.cn
lab head
Donglei Zhang
contact affiliationHuazhong University of Science and Technology
contact emailzhang_donglei@hust.edu.cn
dataset submitter
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Dataset FTP location
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