PXD033499

PXD033499 is an original dataset announced via ProteomeXchange.

Title	Proteome of Staphylococcus aureus biofilm changes signifi-cantly with aging
Description	Staphylococcus aureus is a notorious biofilm-producing pathogen that is frequently isolated from implantable medical device infections. As biofilm ages, it becomes more tolerant to antimicrobial treatment leading to treatment failure and necessitating the costly removal of infected devices. In this study, we investigated what changes occur in the proteome of S. aureus biofilm grown for 3-days and 12-days in comparison with 24 h planktonic showed that proteins associated with bi-osynthetic processes, ABC transporter pathway, virulence proteins, and shikimate kinase pathway were significantly upregulated in 3-day biofilm, while proteins associated with sugar transporter, degradation, and stress response were downregulated. In 12-day biofilms, proteins associated with peptidoglycan biosynthesis, sugar transporters, and stress responses were upregulated, whereas proteins associated with ABC transporters, DNA replication, and adhesion proteins were down-regulated. Furthermore, we observed significant variations in the formation of biofilms result from changes in the level of metabolic activity in the different growth mode of biofilms that could be a significant factor of S. aureus biofilm maturation and persistence. Collectively, potential marker proteins were identified and further characterized to understand their exact role in S. aureus biofilm development which may shed light on possible new therapeutic regimes in the treatment of biofilm-related implant-associated infections.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:57:25.291.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arifur Rahman
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 01:00:23	ID requested
⏵ 1	2022-08-12 00:57:26	announced

Rahman MA, Amirkhani A, Chowdhury D, Mempin M, Molloy MP, Deva AK, Vickery K, Hu H, Biofilm Changes Significantly with Aging. Int J Mol Sci, 23(12):(2022) [pubmed]

submitter keyword: Staphylococcus aureus, biofilms, proteome, TMT, mass spectrometry, aging, metabolic processes, biosynthetic processes, transport systems, stress responses.

Belinda Schiller
contact affiliation	Australian Proteome Analysis Facility, Macquarie University, Sydney NSW 2109, Australia
contact email	belinda.schiller@mq.edu.au
lab head
Arifur Rahman
contact affiliation	Macquarie University
contact email	md-arifur.rahman2@hdr.mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033499

PRIDE project URI

• PRIDE
  1. PXD033499
     1. Label: PRIDE project
     2. Name: Proteome of Staphylococcus aureus biofilm changes signifi-cantly with aging