PXD033470

PXD033470 is an original dataset announced via ProteomeXchange.

Title	Tryptophan C-mannosylation is critical for Plasmodium falciparum transmission Dataset1
Description	C-mannosylation stabilizes proteins bearing a thrombospondin repeat (TSR) domain in metazoans. Here we show that Plasmodium falciparum expresses a DPY19 C-mannosyltransferase in the endoplasmic reticulum and that DPY19-deficiency abolishes C-glycosylation, destabilizes members of the TRAP adhesin family and inhibits transmission to mosquitoes. P. falciparum gametogenesis was imaged in its entirety in four dimensions using lattice light-sheet microscopy. This revealed defects in egress and exflagellation for DPY19 microgametes. While exflagellation was diminished, DPY19 microgametes still fertilized macrogametes, forming ookinetes but these were abrogated for mosquito infection. The gametogenesis defects corresponded with destabilization of MTRAP, which we show is C-mannosylated in P. falciparum, and the ookinete defect was concordant with defective CTRP secretion on the DPY19 background. Genetic complementation of DPY19 restored ookinete infectivity, sporozoite production and C-mannosylation activity. Therefore, tryptophan C-mannosylation by DPY19 in the early secretory pathway ensures TSR protein quality control at two lifecycle stages for successful transmission of the human malaria parasite.
HostingRepository	PRIDE
AnnounceDate	2022-07-06
AnnouncementXML	Submission_2022-07-06_14:49:37.917.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nichollas Scott
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	complex glycosylation
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-27 03:47:26	ID requested
⏵ 1	2022-07-06 14:49:38	announced

Dataset with its publication pending

submitter keyword: Plasmodium falciparum, transmission, glycosylation, gametocyte, gamete, ookinete, sporozoite, DPY19

Justin A. Boddey
contact affiliation	-The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia. -Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia.
contact email	boddey@wehi.edu.au
lab head
Nichollas Scott
contact affiliation	University of Melbourne
contact email	nichollas.scott@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD033470

PRIDE project URI

• PRIDE
  1. PXD033470
     1. Label: PRIDE project
     2. Name: Tryptophan C-mannosylation is critical for Plasmodium falciparum transmission Dataset1