PXD033466

PXD033466 is an original dataset announced via ProteomeXchange.

Title	A proximity labeling method for protein-protein interactions on cell membrane
Description	We introduce antigen-antibody proximity labeling (AAPL), a novel method to map the antigen interaction sites as well as interactors of antibody-targeted proteins. As a proof of concept, AAPL was demonstrated using sodium/potassium transporting ATPase (ATP1A1) and epidermal growth factor receptor 2 (ERBB2)-specific antibodies that were modified with an Fe(III) catalytic probe. Once bound to their target proteins, Fe(III)-induced catalytic oxidation occurred in proximity of the antigen’s epitope. Oxidative proteomic analysis was then used to determine the degree of oxidation, the site of oxidation within the targeted antigen, and the interacting proteins that were in close proximity to the targeted antigen. An AAPL score was generated for each protein yielding the specificity of the oxidation and proximity of the interacting protein to the target antigen. As a final demonstration of its utility, the AAPL approach was applied to map the interactors of liver-intestine-cadherin (CDH17) in colon cancer cells.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_01:10:48.304.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033466
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Qiongyu Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-27 03:45:57	ID requested
⏵ 1	2022-08-12 01:10:49	announced

Li Q, Xie Y, Rice R, Maverakis E, Lebrilla CB, A proximity labeling method for protein-protein interactions on cell membrane. Chem Sci, 13(20):6028-6038(2022) [pubmed]

submitter keyword: Proximity labeling proteomics, antigen-antibody binding, protein-protein interaction, glycosylation

Carlito B Lebrilla
contact affiliation	University of California, Davis Department of Chemistry
contact email	cblebrilla@ucdavis.edu
lab head
Qiongyu Li
contact affiliation	University of California, Davis
contact email	qyuli@ucdavis.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033466

PRIDE project URI

• PRIDE
  1. PXD033466
     1. Label: PRIDE project
     2. Name: A proximity labeling method for protein-protein interactions on cell membrane