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PXD033466

PXD033466 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA proximity labeling method for protein-protein interactions on cell membrane
DescriptionWe introduce antigen-antibody proximity labeling (AAPL), a novel method to map the antigen interaction sites as well as interactors of antibody-targeted proteins. As a proof of concept, AAPL was demonstrated using sodium/potassium transporting ATPase (ATP1A1) and epidermal growth factor receptor 2 (ERBB2)-specific antibodies that were modified with an Fe(III) catalytic probe. Once bound to their target proteins, Fe(III)-induced catalytic oxidation occurred in proximity of the antigen’s epitope. Oxidative proteomic analysis was then used to determine the degree of oxidation, the site of oxidation within the targeted antigen, and the interacting proteins that were in close proximity to the targeted antigen. An AAPL score was generated for each protein yielding the specificity of the oxidation and proximity of the interacting protein to the target antigen. As a final demonstration of its utility, the AAPL approach was applied to map the interactors of liver-intestine-cadherin (CDH17) in colon cancer cells.
HostingRepositoryPRIDE
AnnounceDate2022-08-12
AnnouncementXMLSubmission_2022-08-12_01:10:48.304.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD033466
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterQiongyu Li
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdihydroxylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-27 03:45:57ID requested
12022-08-12 01:10:49announced
Publication List
Li Q, Xie Y, Rice R, Maverakis E, Lebrilla CB, A proximity labeling method for protein-protein interactions on cell membrane. Chem Sci, 13(20):6028-6038(2022) [pubmed]
Keyword List
submitter keyword: Proximity labeling proteomics, antigen-antibody binding, protein-protein interaction, glycosylation
Contact List
Carlito B Lebrilla
contact affiliationUniversity of California, Davis Department of Chemistry
contact emailcblebrilla@ucdavis.edu
lab head
Qiongyu Li
contact affiliationUniversity of California, Davis
contact emailqyuli@ucdavis.edu
dataset submitter
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Dataset FTP location
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