PXD033454

PXD033454 is an original dataset announced via ProteomeXchange.

Title	A Bioorthogonal Chemical Reporter for the Detection and Identification of Protein Lactylation
Description	L-Lactylation is a recently discovered post-translational modification occurring on histone lysine residues to regulate gene expression. However, the substrate scope of lactylation, especially that in non-histone proteins, remains unknown, largely due to the limitations of current methods for analyzing lactylated proteins. Herein, we report an alkynyl-functionalized bioorthogonal chemical reporter, YnLac, for the detection and identification of protein lactylation in mammalian cells. Our in-gel fluorescence and chemical proteomic analyses show that YnLac is metabolically incorporated into lactylated proteins and directly labels known lactylated lysines of histones. We further apply YnLac to the proteome-wide profiling of lactylation, revealing many novel modification sites in non-histone proteins for the first time.
HostingRepository	PRIDE
AnnounceDate	2022-06-23
AnnouncementXML	Submission_2022-06-22_21:28:54.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Peng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-25 04:28:15	ID requested
⏵ 1	2022-06-22 21:28:54	announced

Sun Y, Chen Y, Peng T, A bioorthogonal chemical reporter for the detection and identification of protein lactylation. Chem Sci, 13(20):6019-6027(2022) [pubmed]

submitter keyword: Lactylation, Chemical Reporter, Chemical Proteomics

Tao Peng
contact affiliation	State Key Laboratory of Chemical Oncogenomics, Guangdong Provincial Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China
contact email	tpeng@pku.edu.cn
lab head
Tao Peng
contact affiliation	Peking University Shenzhen Graduate School
contact email	penglab@yeah.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD033454

PRIDE project URI

• PRIDE
  1. PXD033454
     1. Label: PRIDE project
     2. Name: A Bioorthogonal Chemical Reporter for the Detection and Identification of Protein Lactylation