PXD033446

PXD033446 is an original dataset announced via ProteomeXchange.

Title	Functional crosstalk between the cohesin loader and chromatin remodelers
Description	The cohesin complex has crucial roles in many structural and functional aspects of chromosomes including sister chromatid cohesion, genome organization, gene transcription and DNA repair. Cohesin recruitment onto chromosomes requires nucleosome free DNA and a specialized cohesin loader complex comprised of the Scc2 and Scc4 subunits. The cohesin loader, in addition to stimulating cohesin ATP hydrolysis and facilitating topological loading onto DNA, leads cohesin to chromatin receptors such as the RSC chromatin remodeling complex. Here, we explore the cohesin loader-RSC interaction and show that its Scc4 subunit contacts a conserved RSC ATPase motor module. The cohesin loader enhances RSC chromatin remodeling activity in vitro, as well as promoter nucleosome eviction in vivo. These findings provide insight into how the cohesin loader recognizes, as well as influences, the chromatin landscape, with implications for our understanding of human developmental disorders including Cornelia de Lange and Coffin-Siris syndromes.
HostingRepository	PRIDE
AnnounceDate	2022-11-29
AnnouncementXML	Submission_2022-11-29_05:59:42.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AndrewJones
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-25 04:09:02	ID requested
⏵ 1	2022-11-29 05:59:42	announced

Dataset with its publication pending

submitter keyword: Chromatin, Cross-Linking

FrankUhlmann
contact affiliation	The Francis Crick Institute
contact email	Frank.Uhlmann@crick.ac.uk
lab head
AndrewJones
contact affiliation	The Francis Crick Institute
contact email	Andrew.Jones@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD033446

PRIDE project URI

• PRIDE
  1. PXD033446
     1. Label: PRIDE project
     2. Name: Functional crosstalk between the cohesin loader and chromatin remodelers