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PXD033438

PXD033438 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic Analysis of Tomato Under Ebb-and-Flow Subirrigation
DescriptionThe ebb-and-flow subirrigation (EFI) is a water-saving and environment-friendly irrigation method, which can effectively improve water use efficiency and promote plant growth. In this study, we elucidated the effects of ebb-and-flow subirrigation on the protein levels in tomato roots in comparison with top sprinkle irrigation (TSI), and used an integrated approach involving tandem mass tag (TMT) labeling, high performance liquid chromatography (HPLC) fractionation, and mass spectrometry (MS) based analysis. A total of 8,510 quantifiable proteins and 513 dif-ferentially accumulated proteins (DAPs) were identified, of which the expressions of 283 DAPs were up-regulated, and 230 DAPs were down-regulated in the EFI vs TSI treatment comparison. According to proteomic data, we performed a systematic bioinformatics analysis of all identified proteins and DAPs. The DAPs were most significantly associated with the terms “metabolic process”, “anchored component of membrane”, “oxidoreductase activity”, “phenylpropanoid biosynthesis” and “biosynthesis of secondary metabolites” according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment (KEGG) analysis. The 272 DAPs were classified into 12 subcellular components according to their subcellular localizations.
HostingRepositoryiProX
AnnounceDate2022-04-24
AnnouncementXMLSubmission_2022-04-24_20:38:31.719.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMuhammad Moaaz Ali
SpeciesList scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationListTMT6plex reporter fragment
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-24 20:36:33ID requested
12022-04-24 20:38:32announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: irrigation, root-softening, antioxidant, ebb-and-flow, tandem mass tag
Contact List
Faxing Chen
contact affiliationFujian Agriculture and Forestry University, Fuzhou, China
contact emailcfaxing@126.com
lab head
Muhammad Moaaz Ali
contact affiliationFujian Agriculture and Forestry University, Fuzhou, China
contact emailmuhammadmoaazali@yahoo.com
dataset submitter
Full Dataset Link List
iProX dataset URI