PXD033429

PXD033429 is an original dataset announced via ProteomeXchange.

Title	Global bottom-up analysis of wild type and RBM20 knock out rat hearts by LC-TIMS-MSMS
Description	Dilated cardiomyopathy (DCM) is a major risk factor for developing heart failure and is often associated with an increased risk for life-threatening arrhythmia. Although numerous causal genes for DCM have been identified, RNA binding motif 20 (RBM20) remains one of the few splicing factors that, when mutated or genetically ablated, leads to the development of DCM. In this study we sought to identify changes in the cardiac proteome in RBM20 deficient rat hearts using global quantitative proteomics to gain insight into the molecular mechanisms precipitating the development of DCM secondary to RBM20 loss. Our analysis identified changes in titin interacting proteins, as well as mitochondrial enzymes, implicating activation of pathological hypertrophy and mitochondrial dysfunction in DCM development in RBM20 deficient rats. Collectively, our findings provide the first look into changes in the cardiac proteome associated with genetic ablation of RBM20.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:02.212.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eli Larson
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-24 12:50:06	ID requested
1	2023-05-12 11:16:13	announced
⏵ 2	2023-11-14 08:56:02	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Rat, RBM20, Heart, timsTOF Pro

Ying Ge
contact affiliation	Department of Cell and Regenerative Biology, Department of Chemistry, University of Wisconsin-Madison
contact email	ying.ge@wisc.edu
lab head
Eli Larson
contact affiliation	University of Wisconsin-Madison
contact email	ejlarson2@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD033429

PRIDE project URI

• PRIDE
  1. PXD033429
     1. Label: PRIDE project
     2. Name: Global bottom-up analysis of wild type and RBM20 knock out rat hearts by LC-TIMS-MSMS