PXD033427

PXD033427 is an original dataset announced via ProteomeXchange.

Title	Stage II of Chronic Kidney Disease - a Tipping Point?
Description	Background Chronic kidney disease (CKD) is the progressive loss of renal function. Although advances have been made in understanding the progression of CKD, key molecular events in complex pathophysiological mechanisms that mark each stage of renal failure remain largely unknown. Changes in plasma protein profiles in different disease stages are important for identification of early diagnostic markers and potential therapeutic targets. The goal of this study was to determine the molecular profile of each CKD stage (from 1 to 5), aiming to specifically point out markedly expressed or downregulated proteins. Methods We performed a cross-sectional shotgun-proteomic study of plasma proteome across CKD stages and compared them to healthy controls. After sample pooling and heparin-column purification we analysed proteomes from healthy to CKD stage 1 through 5 participants’ plasma by liquid-chromatography/mass-spectrometry. Results We identified 298 proteins in all study groups, as well as several outliers. Our results indicate that key events, that may later affect the course of disease progression and the overall pathophysiological background, are most pronounced in CKD stage 2, with an emphasis on inflammation, lipoprotein metabolism, angiogenesis and tissue regeneration. Conclusions Our data is consistent with multiple previous studies in CKD research, but also uncovers previously unknown CKD mediators and brings forth several novel potential protein-candidates which might have a significant impact on the development of diagnostic and therapeutic strategies for CKD. We hypothesize that CKD stage 2 is the tipping point in disease progression and a suitable point in disease course for the development of therapeutic solutions.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:38:11.387.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruđer Novak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-24 12:46:30	ID requested
⏵ 1	2022-08-12 00:38:12	announced

Grgurevic L, Novak R, Salai G, Hrkac S, Mocibob M, Vojtusek IK, Laganovic M, Stage II of Chronic Kidney Disease-A Tipping Point in Disease Progression? Biomedicines, 10(7):(2022) [pubmed]

submitter keyword: chronic kidney disease, proteomics, inflammation, tissue regeneration, angiogenesis, lipoprotein metabolism, biomarker, plasma, complement, apolipoprotein

Lovorka Grgurević
contact affiliation	Center for Translational and Clinical Research, Department of Proteomics, School of Medicine, University of Zagreb, Zagreb, Croatia
contact email	lovorka.grgurevic@mef.hr
lab head
Ruđer Novak
contact affiliation	School of Medicine, University of Zagreb
contact email	rudjer.novak@mef.hr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033427
     1. Label: PRIDE project
     2. Name: Stage II of Chronic Kidney Disease - a Tipping Point?