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PXD033412

PXD033412 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHepatic urea, creatinine and uric acid metabolism in dairy cows with divergent milk urea concentrations
DescriptionMilk urea concentration is an indicator for dietary nitrogen (N)-supply and urinary nitrogen excretion. Dairy cows with high (HMU) compared to low milk urea (LMU) concentration have greater plasma urea, creatinine and uric acid concentrations, but if the liver metabolism accounts for these differences is unknown. Eighteen HMU and 18 LMU cows were fed either a diet with a low (LP) and normal (NP) crude protein concentration. An N-balance study was performed and a 13C-urea bolus was administered followed by a series of blood samplings. Liver samples were analysed by 2D-gel-based proteomics and real-time RT-PCR. Although HMU cows had a higher urea pool, plasma urea, uric acid, and hippuric acid concentrations, these differences were not associated with increased activation of the hepatic urea cycle or non-urea nitrogen metabolite pathways. Instead, HMU cows had a higher oxidative stress level. Conclusively, other factors than hepatic urea metabolism account for the milk urea concentration. Despite higher plasma urea concentrations on the LP diet, urea cycle mRNA expression was not affected, indicating it is not controlled at transcriptional level. Feeding the LP diet resulted in higher expressions of enzymes catabolizing fatty acids, which is likely due to diminished microbial growth and insufficient energy supply.
HostingRepositoryPRIDE
AnnounceDate2022-09-27
AnnouncementXMLSubmission_2022-09-27_07:15:31.677.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDirk Albrecht
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListNo PTMs are included in the dataset
Instrument5800 TOF/TOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-24 12:26:25ID requested
12022-09-27 07:15:32announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, gel electrophoresis, PCR, purine, nitrogen excretion
Contact List
Dirk Albrecht
contact affiliationInstitut of Microbiology, Univerity of Greifswald
contact emaildirkalbr@uni-greifswald.de
lab head
Dirk Albrecht
contact affiliationEMAU
contact emaildirkalbr@uni-greifswald.de
dataset submitter
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Dataset FTP location
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