⮝ Full datasets listing

PXD033401

PXD033401 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpatial multiomic analysis of synthesis, transport and secretion in the hypothalamus-neurohypophysial system
DescriptionThe cell bodies of hypothalamic magnocellular neurones (MCNs) are confined to the hypothalamic supraoptic nucleus (SON) whereas their axons project to the anatomically discrete posterior pituitary gland (PP). We have taken advantage of this unique anatomical structure to document proteome and phosphoproteome dynamics in neuronal cell bodies and axonal terminals in response to neuronal activation induced by water deprivation (WD). We have found that proteome and phosphoproteome responses to WD are very different between cell bodies and axonal terminals, indicating the need of each cell domain to differentially adapt in response to stimuli. Whilst changes in the proteome and phosphoproteome in the cell body are involved in protein synthesis and cytoskeleton reorganisation, respectively, in the axonal terminals they regulate synaptic vesicle cycle and secretion. Further, by comparison with transcriptome data, we have identified peptides that are not synthesised in the SON, but are present as a consequence of afferent delivery.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:53:50.717.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBenjamin Gillard
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-23 15:40:36ID requested
12023-07-13 03:21:21announced
22023-11-14 09:02:16announced2023-11-14: Updated project metadata.
32024-10-22 05:53:51announced2024-10-22: Updated project metadata.
Publication List
B, á, rez-L, ó, pez S, Mecawi AS, Bryan N, Pau, ž, a AG, Duque VJ, Gillard BT, Murphy D, Greenwood MP, Translational and Posttranslational Dynamics in a Model Peptidergic System. Mol Cell Proteomics, 22(5):100544(2023) [pubmed]
10.1016/j.mcpro.2023.100544;
Keyword List
submitter keyword: SON, Posterior Pituitary, Vasopressin, Proteome, Rat, Phosphoproteome
Contact List
Professor David Murphy
contact affiliationBristol Medical School, University of Bristol
contact emaild.murphy@bristol.ac.uk
lab head
Benjamin Gillard
contact affiliationUniversity of Bristol
contact emailbg14337@bristol.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD033401
PRIDE project URI
Repository Record List
[ + ]