PXD033401 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spatial multiomic analysis of synthesis, transport and secretion in the hypothalamus-neurohypophysial system |
Description | The cell bodies of hypothalamic magnocellular neurones (MCNs) are confined to the hypothalamic supraoptic nucleus (SON) whereas their axons project to the anatomically discrete posterior pituitary gland (PP). We have taken advantage of this unique anatomical structure to document proteome and phosphoproteome dynamics in neuronal cell bodies and axonal terminals in response to neuronal activation induced by water deprivation (WD). We have found that proteome and phosphoproteome responses to WD are very different between cell bodies and axonal terminals, indicating the need of each cell domain to differentially adapt in response to stimuli. Whilst changes in the proteome and phosphoproteome in the cell body are involved in protein synthesis and cytoskeleton reorganisation, respectively, in the axonal terminals they regulate synaptic vesicle cycle and secretion. Further, by comparison with transcriptome data, we have identified peptides that are not synthesised in the SON, but are present as a consequence of afferent delivery. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:53:50.717.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Benjamin Gillard |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-23 15:40:36 | ID requested | |
1 | 2023-07-13 03:21:21 | announced | |
2 | 2023-11-14 09:02:16 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:53:51 | announced | 2024-10-22: Updated project metadata. |
Publication List
B, á, rez-L, ó, pez S, Mecawi AS, Bryan N, Pau, ž, a AG, Duque VJ, Gillard BT, Murphy D, Greenwood MP, Translational and Posttranslational Dynamics in a Model Peptidergic System. Mol Cell Proteomics, 22(5):100544(2023) [pubmed] |
10.1016/j.mcpro.2023.100544; |
Keyword List
submitter keyword: SON, Posterior Pituitary, Vasopressin, Proteome, Rat, Phosphoproteome |
Contact List
Professor David Murphy |
contact affiliation | Bristol Medical School, University of Bristol |
contact email | d.murphy@bristol.ac.uk |
lab head | |
Benjamin Gillard |
contact affiliation | University of Bristol |
contact email | bg14337@bristol.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033401
- Label: PRIDE project
- Name: Spatial multiomic analysis of synthesis, transport and secretion in the hypothalamus-neurohypophysial system