PXD033401

PXD033401 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Spatial multiomic analysis of synthesis, transport and secretion in the hypothalamus-neurohypophysial system
Description	The cell bodies of hypothalamic magnocellular neurones (MCNs) are confined to the hypothalamic supraoptic nucleus (SON) whereas their axons project to the anatomically discrete posterior pituitary gland (PP). We have taken advantage of this unique anatomical structure to document proteome and phosphoproteome dynamics in neuronal cell bodies and axonal terminals in response to neuronal activation induced by water deprivation (WD). We have found that proteome and phosphoproteome responses to WD are very different between cell bodies and axonal terminals, indicating the need of each cell domain to differentially adapt in response to stimuli. Whilst changes in the proteome and phosphoproteome in the cell body are involved in protein synthesis and cytoskeleton reorganisation, respectively, in the axonal terminals they regulate synaptic vesicle cycle and secretion. Further, by comparison with transcriptome data, we have identified peptides that are not synthesised in the SON, but are present as a consequence of afferent delivery.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:53:50.717.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Gillard
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-23 15:40:36	ID requested
1	2023-07-13 03:21:21	announced
2	2023-11-14 09:02:16	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:53:51	announced	2024-10-22: Updated project metadata.

Publication List

B, á, rez-L, ó, pez S, Mecawi AS, Bryan N, Pau, ž, a AG, Duque VJ, Gillard BT, Murphy D, Greenwood MP, Translational and Posttranslational Dynamics in a Model Peptidergic System. Mol Cell Proteomics, 22(5):100544(2023) [pubmed]
10.1016/j.mcpro.2023.100544;

B, á, rez-L, ó, pez S, Mecawi AS, Bryan N, Pau, ž, a AG, Duque VJ, Gillard BT, Murphy D, Greenwood MP, Translational and Posttranslational Dynamics in a Model Peptidergic System. Mol Cell Proteomics, 22(5):100544(2023) [pubmed]

10.1016/j.mcpro.2023.100544;

Keyword List

submitter keyword: SON, Posterior Pituitary, Vasopressin, Proteome, Rat, Phosphoproteome

submitter keyword: SON, Posterior Pituitary, Vasopressin, Proteome, Rat, Phosphoproteome

Contact List

Professor David Murphy
contact affiliation	Bristol Medical School, University of Bristol
contact email	d.murphy@bristol.ac.uk
lab head
Benjamin Gillard
contact affiliation	University of Bristol
contact email	bg14337@bristol.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD033401
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD033401

PRIDE project URI

Repository Record List

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