PXD033397

PXD033397 is an original dataset announced via ProteomeXchange.

Title	Taf2 mediates DNA binding of Taf14
Description	The assembly and function of the yeast general transcription factor TFIID complex requires specific contacts between its Taf14 and Taf2 subunits, however, the mechanism underlying these contacts remains unclear. Here, we determined the molecular and structural basis by which the YEATS and ET domains of Taf14 bind to the C-terminal tail of Taf2 and identified a unique DNA-binding activity of the linker region connecting the two domains. We show that in the absence of ligands the linker region of Taf14 is occluded by the surrounding domains, and therefore the DNA binding function of Taf14 is autoinhibited. Binding of Taf2 promotes a conformational rearrangement in Taf14, resulting in a release of the linker for the engagement with DNA and the nucleosome. Genetic in vivo data indicate that the association of Taf14 with both Taf2 and DNA is essential for transcriptional regulation. Our findings provide a basis for deciphering the role of individual TFIID subunits in mediating gene transcription.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:29:27.438.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Ebmeier
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-23 15:36:03	ID requested
⏵ 1	2022-08-12 00:29:28	announced

Klein BJ, Feigerle JT, Zhang J, Ebmeier CC, Fan L, Singh RK, Wang WW, Schmitt LR, Lee T, Hansen KC, Liu WR, Wang YX, Strahl BD, Anthony Weil P, Kutateladze TG, Taf2 mediates DNA binding of Taf14. Nat Commun, 13(1):3177(2022) [pubmed]

submitter keyword: Taf2 mediates DNA binding of Taf14

Professor Tatiana Kutateladze
contact affiliation	Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
contact email	tatiana.kutateladze@cuanschutz.edu
lab head
Christopher Ebmeier
contact affiliation	University of Colorado Boulder
contact email	christopher.ebmeier@colorado.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033397

PRIDE project URI

• PRIDE
  1. PXD033397
     1. Label: PRIDE project
     2. Name: Taf2 mediates DNA binding of Taf14