PXD033395

PXD033395 is an original dataset announced via ProteomeXchange.

Title	Differential gene expression of cells located in the top and bottom of yeast colonies
Description	Saccharomyces cerevisiae colonies were grown on synthetic minimal media containing 1% glucose. The top layer of cells was scraped off using a custom plastic guide and a cell scraper. The remaining bottom layer of cells was then washed off with water and proteomics samples were prepared from both layers separately. Label-free quantification by DIA-SWATH was used to assess differences in gene expression between top and bottom layers.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:22.290.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Kamrad
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-23 15:33:41	ID requested
1	2023-02-13 04:28:39	announced
⏵ 2	2023-11-14 08:56:22	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: DIA, SWATH, colony,yeast

Markus Ralser
contact affiliation	Charité Universitätsmedizin Berlin, Institute of Biochemistry
contact email	markus.ralser@charite.de
lab head
Stephan Kamrad
contact affiliation	The Francis Crick Institute
contact email	stephan.kamrad@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD033395

PRIDE project URI

• PRIDE
  1. PXD033395
     1. Label: PRIDE project
     2. Name: Differential gene expression of cells located in the top and bottom of yeast colonies