PXD033393

PXD033393 is an original dataset announced via ProteomeXchange.

Title	Fungal-bacterial co-infection of macrophages reveals global reprogramming and adaptation during infection
Description	In this study, we use mass spectrometry (MS)-based proteomics to define the host response under single and dual infection states of macrophages with C. neoformans and K. pneumoniae to define critical responses of each biological system. This high-resolution comparative analysis illustrates how protein abundance deviates during a transition from an acute to chronic infectious state with exposure to additional microbial stimuli. We reveal global changes upon infection followed by pathogen-specific host response signatures. Additionally, we define regulatory changes within C. neoformans as the fungi adapts to the host environment and stabilizes prior to further disruption in the presence of chronic bacterial infection. We validate our findings with host cytokine detection and phenotypic profiling of the fungi throughout the host and bacterial exposures. Overall, our study provides an in-depth analysis of cross-kingdom protein level changes during macrophage infection. This information provides new insight into fungal modulation of the immune response, including a stabilization and adaptation of the host and fungi during a chronic infection, which is disrupted upon chronic co-infection with a bacterial pathogen.
HostingRepository	PRIDE
AnnounceDate	2022-06-30
AnnouncementXML	Submission_2022-06-30_12:05:19.992.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Cryptococcus neoformans var. grubii H99; NCBI TaxID: 235443; scientific name: Klebsiella pneumoniae; NCBI TaxID: 573;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-23 15:31:40	ID requested
⏵ 1	2022-06-30 12:05:20	announced

Dataset with its publication pending

submitter keyword: Co-infection, Quantitative proteomics, Macrophages, Pathogenesis, Cryptococcus neoformans, Klebsiella pneumoniae

Jennifer Geddes-McAlister
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD033393

PRIDE project URI

• PRIDE
  1. PXD033393
     1. Label: PRIDE project
     2. Name: Fungal-bacterial co-infection of macrophages reveals global reprogramming and adaptation during infection