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PXD033387

PXD033387 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInteractions between paralogous bacterial enhancer binding proteins enable metal-dependent regulation of alternative nitrogenases in Azotobacter vinelandii
DescriptionAll diazotrophic bacteria and archaea isolated so far utilise a nitrogenase enzyme containing molybdenum in the active site co-factor to fix atmospheric dinitrogen to ammonia. However, in addition to the Mo-dependent nitrogenase, some nitrogen-fixing prokaryotes also express genetically distinct alternative nitrogenase isoenzymes, namely the V-dependent and Fe-only nitrogenases respectively. Nitrogenase isoenzymes are expressed hierarchically according to metal availability and catalytic efficiency. In proteobacteria, this hierarchy is maintained via stringent transcriptional regulation of gene clusters by dedicated bacterial enhancer binding proteins (bEBPs). The model diazotroph Azotobacter vinelandii contains two paralogs of the vanadium nitrogenase activator VnfA (henceforth, VnfA1), designated VnfA2 and VnfA3, with unknown functions. Here we demonstrate that the VnfA1 and VnfA3 bEBPs bind to the same target promoters in the Azotobacter vinelandii genome and co-activate a subset of genes in the absence of V, including the structural genes for the Fe only nitrogenase. Co-activation is inhibited by the presence of V and is dependent on an accessory protein VnfZ that is co-expressed with VnfA3. Our studies uncover a plethora of interactions between bEBPs required for nitrogen fixation, revealing unprecedented potential for fine tuning expression of alternative nitrogenases in response to metal availability.
HostingRepositoryPRIDE
AnnounceDate2022-06-23
AnnouncementXMLSubmission_2022-06-23_07:48:31.444.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD033387
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCarlo de Oliveira Martins
SpeciesList scientific name: Azotobacter vinelandii DJ; NCBI TaxID: 322710;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-23 15:27:33ID requested
12022-06-23 07:48:31announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Azotobacter, alternative nitrogenases, Co-IP, LC-MSMS
Contact List
Ray Dixon
contact affiliationGroup Leader, Department of Molecular Microbiology
contact emailRay.Dixon@jic.ac.uk
lab head
Carlo de Oliveira Martins
contact affiliationJohn Innes Centre
contact emailCarlo.Martins@jic.ac.uk
dataset submitter
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