PXD033381 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Neuromelanin granules of the substantia nigra: proteomic profile provides links to tyrosine hydroxylase, stress granules and lysosomes |
Description | Neuromelanin is a black-brownish pigment, present in so-called neuromelanin granules (NMGs) in the cell bodies of dopaminergic neurons in the substantia nigra (SN) pars compacta. These neurons are lost in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Although it is known that lipids, proteins, and environmental toxins accumulate in NMGs, the function of NMGs has not yet been finally clarified as well as their origin and the synthesis of neuromelanin. We therefore isolated NMGs and surrounding SN tissue from control patients by laser microdissection and analyzed the proteomic profile by tandem mass spectrometry. With our improved workflow, we were able to 1) strengthen the regularly reported link between NMGs and lysosomes, 2) detect tyrosine hydroxylase to be highly abundant in NMGs, which may be related to neuromelanin synthesis and 3) indicate a yet undescribed link between stress granules (SGs) and NMGs. Based on our findings, we cautiously hypothesize, that SGs may be the origin of NMGs or form in close proximity to them, potentially due to the oxidative stress caused by neuromelanin-bound metals. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:25:26.998.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maximilian Wulf |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-23 15:22:41 | ID requested | |
1 | 2023-02-14 02:47:40 | announced | |
⏵ 2 | 2023-11-14 08:25:29 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wulf M, Barkovits K, Schork K, Eisenacher M, Riederer P, Gerlach M, Eggers B, Marcus K, Neuromelanin granules of the substantia nigra: proteomic profile provides links to tyrosine hydroxylase, stress granules and lysosomes. J Neural Transm (Vienna), 129(10):1257-1270(2022) [pubmed] |
Keyword List
submitter keyword: laser microdissection, stress granules, mass spectrometry, substantia nigra pars compacta, neuromelanin synthesis,Neuromelanin granules |
Contact List
Katrin Marcus |
contact affiliation | Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, Bochum, Germany |
contact email | katrin.marcus@ruhr-uni-bochum.de |
lab head | |
Maximilian Wulf |
contact affiliation | Medizinisches Proteom-Center, Ruhr-University Bochum |
contact email | Maximilian.Wulf@ruhr-uni-bochum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033381
- Label: PRIDE project
- Name: Neuromelanin granules of the substantia nigra: proteomic profile provides links to tyrosine hydroxylase, stress granules and lysosomes